The impact of bitter taste receptor genetics on culturable bacteria in chronic rhinosinusitis
The impact of bitter taste receptor genetics on culturable bacteria in chronic rhinosinusitis
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DOI：
10.4193/rhin16.181
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发表时间：
2017-03-01
期刊：
影响因子：
7.2
通讯作者：
Harvey, Richard J.
中科院分区：
文献类型：
--
作者：
Rom, Darren I.;Christensen, Jenna M.;Harvey, Richard J.
背景：口腔外苦味受体与先天细菌防御机制有关。T2R38功能的遗传变异已被证明与上呼吸道感染和慢性犀牛鼻窦炎（CRS）易感性相关。我们试图独立评估苦味受体基因型对鼻窦中可培养细菌存在的影响。方法：对接受手术的CRS患者进行横断面分析。在手术时送鼻中金属拭子进行微生物学评估。取粘膜活检并送去进行苦味受体基因型分析。对tas2r38基因的3个多态性进行测序，确定了超味觉型（PAV/PAV）、非味觉型（AVI/AVI）和杂合表达型（PAV/AVI）。比较了苦味受体基因型中可培养生物和常见病原菌的存在情况。结果：25例患者（年龄52.4±18.28岁，51%为女性）被评估。超级味觉者占16%，不味觉者占24%，杂合表达者占48%。48%的患者培养出病原体；革兰氏阳性32%，革兰氏阴性20%，金黄色葡萄球菌28%，铜绿假单胞菌12%。非味觉基因型可预测病原体的定植。组织嗜酸性粒细胞增多（bbb10 /HPF）占48%。结论：即使在一小部分CRS患者样本中，非味觉者T2R38基因型似乎也能预测手术时鼻窦腔内是否存在可培养细菌。与患者更容易被“感染”的基因联系是可能的。
Background: Extra-oral bitter taste receptors have been associated with innate bacterial defence mechanisms. Genetic variation in T2R38 functionality has been shown to be associated with susceptibility to upper respiratory tract infections and chronic rhino sinusitis (CRS). We sought to independently assess the influence of bitter taste receptor genotype on the presence of culturable bacteria in the sinuses.Methodology: A cross-sectional analysis of patients with CRS undergoing surgery was performed. Middle meatal nasal swabs were sent for microbiological evaluation at the time of the procedure. Mucosal biopsies were taken and sent for bitter taste receptor genotype analysis. Sequencing of 3 polymorphisms in theTAS2R38 gene was performed to identify genotypes as super tasters (PAV/PAV), non-tasters (AVI/AVI) or heterozygous expression (PAV/AVI). The presence of culturable organisms and common pathogens were compared with bitter taste receptor genotypes.Results: 25 patients (age 52.4 +/- 18.28 years, 51% female) were assessed. Super-tasters comprised 16% of the group, 24% were non-tasters and 48% had heterozygous expression. A cultured pathogen was grown in 48% of patients; 32% gram-positive, 20% gram-negative, 28% grew Staphylococcus aureus and 12% Pseudomonas aeruginosa. A non-taster genotype was predictive of colonised pathogens. Tissue eosinophilia (>10/HPF) was seen in 48%.Conclusion: Even in a small sample of patients with CRS, non-taster T2R38 genotype appears to predict the presence of culturable bacteria colonising the sinus cavity at the time of surgery for their condition. A genetic link to patients more likely to become 'infected' is likely.