Phylotypes related to Ruminococcus bromii are abundant in the large bowel of humans and increase in response to a diet high in resistant starch
Phylotypes related to Ruminococcus bromii are abundant in the large bowel of humans and increase in response to a diet high in resistant starch
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DOI：
10.1111/j.1574-6941.2008.00527.x
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发表时间：
2008-12-01
影响因子：
4.2
通讯作者：
McOrist, Alexandra L.
中科院分区：
文献类型：
--
作者：
Abell, Guy C. J.;Cooke, Caroline M.;McOrist, Alexandra L.
为了进一步了解含有高水平纤维的饮食如何预防结直肠癌，我们在一项随机交叉干预研究中研究了高非淀粉多糖（NSP）或高NSP加抗性淀粉（RS）饮食对46名健康成人粪便微生物群落组成的影响。使用16 S rRNA基因片段的变性梯度凝胶电泳（DGGE）检查细菌种群的变化。细菌谱显示响应于RS和NSP饮食的消耗的变化[相似性分析（ANOSIM）：R=0.341-0.507，P < 0.01]。一些不同的DGGE条带强度增加，在响应饮食干预归因于尚未培养的细菌密切相关的瘤胃球菌溴。实时荧光定量PCR检测特异性R. Bromii组应用于来自饮食研究的粪便样品，并且发现当个体食用其正常饮食时，该组包括总群落的显著比例（总16 S rRNA基因丰度的4.4 +/-2.6%），并且在RS而不是NSP饮食干预下，数量显著增加（+/-67%，P < 0.05）。本研究表明，R.与溴有关的细菌在人体中大量存在，并且在大肠中复杂碳水化合物的发酵中可能是重要的。
To further understand how diets containing high levels of fibre protect against colorectal cancer, we examined the effects of diets high in nonstarch polysaccharides (NSP) or high in NSP plus resistant starch (RS) on the composition of the faecal microbial community in 46 healthy adults in a randomized crossover intervention study. Changes in bacterial populations were examined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Bacterial profiles demonstrated changes in response to the consumption of both RS and NSP diets [analysis of similarities (ANOSIM): R=0.341-0.507, P < 0.01]. A number of different DGGE bands with increased intensity in response to dietary intervention were attributed to as-yet uncultivated bacteria closely related to Ruminococcus bromii. A real-time PCR assay specific to the R. bromii group was applied to faecal samples from the dietary study and this group was found to comprise a significant proportion of the total community when individuals consumed their normal diets (4.4 +/- 2.6% of total 16S rRNA gene abundance) and numbers increased significantly (+/- 67%, P < 0.05) with the RS, but not the NSP, dietary intervention. This study indicates that R. bromii-related bacteria are abundant in humans and may be significant in the fermentation of complex carbohydrates in the large bowel.