Gene regulation by sense-antisense overlap of polyadenylation signals
Gene regulation by sense-antisense overlap of polyadenylation signals
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DOI：
10.1261/rna.1608909
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发表时间：
2009-06-01
期刊：
影响因子：
4.5
通讯作者：
Carmichael, Gordon G.
中科院分区：
文献类型：
--
作者：
Gu, Rui;Zhang, Zuo;Carmichael, Gordon G.
我们在这里表明，从会聚转录单位的基因的表达可以通过在重叠的多聚腺苷酸化信号的区域中形成双链RNA（dsRNA）来调节。采用的模型系统是小鼠多瘤病毒。多瘤病毒的早期和晚期基因从环状病毒基因组的相反链转录。在感染后的早期，早期基因主要表达。晚期基因表达在DNA复制开始时显著增加，此时晚期初级转录物的多聚腺苷酸化中的主要缺陷产生作为成熟晚期mRNA的前体的多基因组RNA。嵌入这些晚期前mRNA中的是与早期RNA互补的序列，其通过dsRNA的A至I编辑下调早期基因表达。在该系统中，缺陷性多聚腺苷酸化以及因此产生的多基因组晚期RNA取决于上下文，也许还取决于与早期转录物3 '末端重叠的poly（A）信号的A到I编辑。
We show here that expression of genes from convergent transcription units can be regulated by the formation of double-stranded RNA ( dsRNA) in the region of overlapping polyadenylation signals. The model system employed is the mouse polyomavirus. The early and late genes of polyomavirus are transcribed from opposite strands of the circular viral genome. At early times after infection, the early genes are expressed predominantly. Late gene expression increases dramatically upon the onset of DNA replication, when a major defect in polyadenylation of the late primary transcripts generates multigenomic RNAs that are precursors to the mature late mRNAs. Embedded in these late pre-mRNAs are sequences complementary to the early RNAs that act to down-regulate early gene expression via A-to-I editing of dsRNAs. In this system, the defective polyadenylation, and consequently the production of multigenomic late RNAs, depends on the context, and perhaps also, on the A-to-I editing of the poly(A) signal that overlaps the 3'-end of early transcripts.