Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057.
Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057.
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乙烯通过调节乙烯响应因子 057 的表达来积极调节葡萄的耐冷性
DOI：
10.1038/srep24066
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发表时间：
2016-04-04
影响因子：
4.6
通讯作者：
Xin H
中科院分区：
文献类型：
--
作者：
Sun X;Zhao T;Gan S;Ren X;Fang L;Karungo SK;Wang Y;Chen L;Li S;Xin H
乙烯(ET)是一种气态植物激素，在植物的生物和非生物胁迫反应中发挥重要作用。然而，ET在不同物种的耐冷性中的作用是不同的。本研究表明，低温促进了葡萄体内ET的释放。外源1-氨基环丙烷-1-羧酸盐处理提高了葡萄的耐冷性。相比之下，ET生物合成抑制剂氨基乙氧基乙烯基甘氨酸的应用降低了葡萄的耐寒性。这一发现表明，ET对葡萄的冷胁迫反应有积极的影响。ET信号转导下游基因VaERF057的表达受低温强烈诱导。VaERF057的过表达也提高了拟南芥的耐冷性。在低温处理下，转基因植株的丙二醛含量低于野生型植株，超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性高于野生型植株。RNA-Seq结果表明，在VaERF057过表达的转基因株系中，CBF1-3等32个逆境相关基因表达上调。酵母单杂交结果进一步证明VaERF057能与GCC盒和DRE基序特异结合。因此，VaERF057可能通过与GCC盒或DRE元件相互作用，直接调控其逆境反应基因的表达。我们的工作证实，ET通过调节VaERF057的表达对葡萄的耐冷性起到积极的调节作用。
Ethylene (ET) is a gaseous plant hormone that plays essential roles in biotic and abiotic stress responses in plants. However, the role of ET in cold tolerance varies in different species. This study revealed that low temperature promotes the release of ET in grapevine. The treatment of exogenous 1-aminocyclopropane-1-carboxylate increased the cold tolerance of grapevine. By contrast, the application of the ET biosynthesis inhibitor aminoethoxyvinylglycine reduced the cold tolerance of grapevine. This finding suggested that ET positively affected cold stress responses in grapevine. The expression ofVaERF057, an ET signaling downstream gene, was strongly induced by low temperature. The overexpression ofVaERF057also enhanced the cold tolerance ofArabidopsis. Under cold treatment, malondialdehyde content was lower and superoxide dismutase, peroxidase, and catalase activities were higher in transgenic lines than in wild-type plants. RNA-Seq results showed that 32 stress-related genes, such asCBF1-3, were upregulated inVaERF057-overexpressing transgenic line. Yeast one-hybrid results further demonstrated thatVaERF057specifically binds to GCC-box and DRE motifs. Thus,VaERF057may directly regulate the expression of its target stress-responsive genes by interacting with a GCC-box or a DRE element. Our work confirmed that ET positively regulates cold tolerance in grapevine by modulating the expression ofVaERF057.