Uptake of oligodeoxyribonucleotides by lymphoid cells is heterogeneous and inducible.
Uptake of oligodeoxyribonucleotides by lymphoid cells is heterogeneous and inducible.
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淋巴细胞对寡脱氧核糖核苷酸的摄取是异质的且可诱导的。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
A. Steinberg
中科院分区：
文献类型：
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作者：
A. Krieg;F. Gmelig‐Meyling;M. Gourley;W. Kisch;L. Chrisey;A. Steinberg
使用内部放射性标记和荧光素-5-异硫氰酸酯（FITC）标记的寡核苷酸在培养的小鼠脾脏和淋巴结细胞中研究了寡核苷酸摄取。通过流式细胞术和细胞表面分子的抗体染色来区分拟棘球蚴亚群。大约5%的新鲜淋巴样细胞摄取大量的寡核苷酸。如果细胞在与标记的寡核苷酸孵育之前培养至少24小时，则摄取寡核苷酸的B细胞的百分比增加5倍，如果细胞预培养48小时，则增加10倍。培养的CD 4+和CD 8 + T细胞具有相似的寡核苷酸摄取，其小于培养的B细胞中的三分之一，但CD 4-CD 8- T细胞具有比B细胞更高的摄取寡核苷酸的细胞百分比。T细胞或B细胞促分裂剂分别引起T细胞或B细胞中寡核苷酸摄取显著增加。寡核苷酸的摄取可以被抑制，只有部分与竞争对手的DNA。为了区分细胞膜结合的寡核苷酸和细胞内的寡核苷酸，将细胞在酸性甘氨酸缓冲液（其去除大部分表面寡核苷酸）中洗涤。这表明大部分寡核苷酸是细胞内的。我们的结论是，寡核苷酸的摄取是相当异质性的培养细胞之间，这种摄取是诱导有丝分裂原。这些数据对于体外实验的设计和解释以及使用反义寡核苷酸的体内治疗的规划可能是重要的。
Oligonucleotide uptake was studied in cultured murine spleen and lymph node cells using internally radiolabeled and fluorescein-5-isothiocyanate (FITC)-labeled oligonucleotides. Lymphoid subpopulations were distinguished by flow cytometry and staining with antibodies to cell-surface molecules. Approximately 5% of fresh lymphoid cells take up substantial amounts of oligonucleotide. The percentage of B cells that take up oligonucleotide increased fivefold if cells were cultured for at least 24 hr prior to incubation with labeled oligonucleotides, and increased 10-fold if cells were precultured for 48 hr. T-cell uptake changed very little in culture. Cultured CD4+ and CD8+ T cells had similar oligonucleotide uptake that was less than one-third of that in cultured B cells, but CD4-CD8- T cells had a higher percentage of cells taking up oligonucleotide than did B cells. T- or B-cell mitogens caused markedly increased oligonucleotide uptake in T or B cells, respectively. Oligonucleotide uptake could be inhibited only partially with competitor DNA. To distinguish between cell membrane-bound and intracellular oligonucleotide, cells were washed in acid glycine buffer (which removes most surface oligonucleotide). This demonstrated that most of the oligonucleotide was intracellular. We conclude that oligonucleotide uptake is quite heterogeneous among cultured cells, and that this uptake is inducible by mitogens. These data may be important for the design and interpretation of in vitro experiments, and for the planning of in vivo therapy with antisense oligonucleotides.
通过肌细胞生成素 mRNA 的反义寡脱氧核糖核苷酸高度特异性抑制 IGF-I 刺激的分化。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Florini,JR;Ewton,DZ
通讯作者：
Ewton,DZ