Effects of crowding in larval Aedes aegypti, using proportionally reduced experimental universes.
Effects of crowding in larval Aedes aegypti, using proportionally reduced experimental universes.
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使用按比例缩小的实验宇宙，研究埃及伊蚊幼虫的拥挤效应。
DOI：
10.1093/aesa/64.1.26
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
P. Barbosa
中科院分区：
文献类型：
--
作者：
N. Greenough;T. Peters;P. Barbosa
采用最佳饲养技术对埃及伊蚊（Aedes aegypti（L.）建议。为了促进高幼虫密度的研究，试图确定使用按比例缩小的实验宇宙的可行性。用于确定最佳发育的标准是存活率、化蛹率和蛹重。
实验I测试了与先前确定的40只幼虫/80 ml的标准成比例的13只幼虫/26 ml和7只幼虫/14 ml的减少的实验宇宙。在所有方面的标准和宇宙与13幼虫是相同的。分别有40只和13只幼虫的宇宙与有7只幼虫的宇宙之间的蛹重量存在差异，尽管化蛹开始、化蛹率或总存活率没有显着差异。
使用与实验I中相同的标准，第二个实验试图测试密度水平是否为13只幼虫/26毫升的比例减少的总体的4倍和10倍，是否相当于标准的4倍和10倍。可比较的密度没有显著差异，即，4倍标准品与4倍还原当量没有差异，10倍标准品与10倍还原当量没有差异，尽管每对之间存在显著差异。
得出的结论是，按比例减少饲养方法可用于研究参数的高度拥挤的人口以前抑制，因为需要过多的时间来处理大量的幼虫。
The use of an optimal rearing technique in the investigation and evaluation of crowded and undercrowded experimental populations of Aedes aegypti (L.) is suggested. To facilitate the study of high larval densities an attempt was made to determine the feasibility of using proportionally reduced experimental universes. The criteria used to determine optimal development were percent survival, pupation rate, and pupal weight.
Experiment I tested reduced experimental universes of 13 larvae/26 ml and 7 larvae/14 ml proportional to a previously determined standard of 40 larvae/80 ml. In all respects the standard and the universe with 13 larvae were identical. There was a difference in pupal weights between the universes with 40 and 13 larvae, respectively, and the universe with 7 larvae, although there was no significant difference in either initiation of pupation, pupation rate, or overall survival.
Using the same criteria as in experiment I, the 2nd experiment attempted to test whether density levels 4 and 10 times that of the proportionally reduced universe of 13 larvae/26 ml would be equivalent to 4 and 10 times the standard. The comparable densities did not differ significantly, i.e., 4 times the standard did not differ from 4 times the reduced equivalent, and 10 times the standard did not differ from 10 times the reduced equivalent, although there was a significant difference between each pair.
It was concluded that the proportionally reduced rearing method could be employed for studying parameters of highly crowded populations previously inhibitive because of the excessive time required to handle large numbers of larvae.