Localization of connective tissue growth factor in human uterine tissues.
Localization of connective tissue growth factor in human uterine tissues.
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结缔组织生长因子在人体子宫组织中的定位。
DOI：
10.1093/molehr/6.12.1093
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发表时间：
2000
影响因子：
4
通讯作者：
Brigstock,DR
中科院分区：
文献类型：
--
作者：
Uzumcu,M;Homsi,MF;Ball,DK;Coskun,S;Jaroudi,K;Hollanders,JM;Brigstock,DR
结缔组织生长因子(CTGF)是近年来发现的一种可与成纤维细胞和平滑肌细胞结合的肝素有丝分裂原。本研究的目的是通过免疫组织化学和Northern印迹分析来研究人子宫组织中CTGF的产生。对于免疫组织化学，用一种针对人CTGF第81-94残基的高度特异的亲和力纯化的多克隆抗体对福尔马林固定的人增殖期(n=5)、分泌早期(n=5；15-19天)、分泌中期(n=5；20-23天)、分泌晚期(n=5；24-28天)子宫内膜和蜕膜(n=5)组织进行染色。子宫肌层(n=5)和子宫肌瘤(n=5)组织行CTGF免疫组织化学染色。增生期子宫内膜上皮细胞和血管内皮细胞CTGF免疫反应呈强阳性，间质细胞CTGF蛋白呈阴性或弱阳性。在整个分泌期，CTGF在子宫内膜上皮细胞和内皮细胞中均有表达。间质细胞仅在分泌早、中期的水肿区和分泌晚期的蜕膜区有较强的CTGF免疫反应。妊娠期蜕膜、子宫内膜上皮和内皮细胞均呈CTGF免疫反应阳性。在子宫肌层和子宫肌瘤标本中，CTGF免疫反应仅见于内皮细胞。2例正常子宫和6例子宫肌瘤的人CTGFc DNA探针Northern印迹结果显示，只有一条2.4kb的转录本。本研究首次证实了CTGF基因在人子宫组织中的表达及其编码蛋白的定位。CTGF的细胞和周期特异性定位支持该分子在月经周期和妊娠期间调节子宫细胞生长、迁移和/或基质产生方面的作用。
Connective tissue growth factor (CTGF) is a recently described heparin-binding mitogen for fibroblasts and smooth muscle cells. The aim of this study was to investigate the production of CTGF by human uterine tissues using immunohistochemical and Northern blotting analyses. For immunohistochemistry, formalin-fixed human proliferative (n= 5), early secretory (n= 5; days 15–19), mid-secretory (n= 5; days 20–23), late secretory (n= 5; days 24–28) endometrial, and decidual (n= 5) tissues were stained using a highly specific affinity-purified polyclonal antibody raised against residues 81–94 of human CTGF. Myometrial (n= 5) and leiomyoma (n= 5) tissues were also used for CTGF immunochemistry. In proliferative endometrium, epithelial and vascular endothelial cells showed strong CTGF immunoreactivity, whereas stromal cells were negative or only weakly positive for the CTGF protein. Throughout the entire secretory stage, CTGF was detected in epithelial and endothelial cells of endometrium. Stromal cells showed strong immunoreactivity to CTGF only in oedematous areas for early and mid-secretory endometrium, and in decidualized regions of late secretory endometrium. During pregnancy, the decidual, epithelial and endothelial cells of the endometrium were all immunoreactive to CTGF. In myometrial and leiomyoma samples, CTGF immunoreactivity was found only in the endothelial cells. Northern blotting of mRNA from normal uterus (n= 2) or leiomyoma (n= 6) using a 320 bp humanCTGFcDNA probe revealed a single 2.4 kb transcript. This study is the first to demonstrateCTGFgene expression and localization of its encoded protein in human uterine tissues. The cell- and cycle-specific localization of CTGF support a role for this molecule in regulating aspects of uterine cell growth, migration, and/or matrix production during the menstrual cycle and pregnancy.