Differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines.
Differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines.
复制标题
转化成纤维细胞和癌细胞系中乳头状瘤病毒早期基因表达的差异调节。
作者：
E. Kleiner;W. Dietrich;H. Pfister
用放线菌酮处理牛乳头瘤病毒（BPV）1转化的小鼠成纤维细胞，在蛋白质合成抑制1小时后，病毒转录物的量增加了10倍。北方印迹显示RNA模式无质的变化。核运行实验显示放线菌酮处理后比转录活性增加约7倍。BPV 1 mRNA的半衰期是未处理对照的两倍。这些结果表明，RNA的合成和病毒RNA的降解是由不稳定的蛋白质控制。环己酰亚胺刺激原来是独立的BPV 1 E2基因活性，增强病毒转录。放线菌酮处理对宫颈癌源性HeLa和C4 - 1细胞中人乳头瘤病毒（HPV）18转录物的量没有影响。宫颈癌细胞系SiHa中HPV 16的转录同样不受影响。在转化的成纤维细胞和癌源性细胞的转录的差异调节，以及恶性转化的意义进行了讨论。
Treatment of bovine papilloma virus (BPV) 1‐transformed mouse fibroblasts with cycloheximide led to a 10‐fold increase in the amount of viral transcripts, after as little as 1 h of protein synthesis inhibition. Northern blots revealed no qualitative changes in the RNA pattern. Nuclear run‐on experiments showed about a 7‐fold increase in specific transcriptional activity after cycloheximide treatment. The half‐life of BPV1 mRNA was twice as long as in untreated controls. These results indicate that both RNA synthesis and degradation of viral RNA are controlled by labile proteins. Cycloheximide stimulation turned out to be independent of the BPV1 E2 gene activity which enhances viral transcription. Cycloheximide treatment had no effect on the amount of human papilloma virus (HPV) 18 transcripts in cervical carcinoma derived HeLa and C4‐1 cells. Transcription of HPV16 in the cervical carcinoma line SiHa was likewise unaffected. The differential regulation of transcription in transformed fibroblasts and cancer‐derived cells, and the significance for malignant conversion are discussed.