Genetic variation within and among populations of a dominant desert tree Haloxylon ammodendron (Amaranthaceae) in China.
Genetic variation within and among populations of a dominant desert tree Haloxylon ammodendron (Amaranthaceae) in China.
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DOI：
10.1093/aob/mci171
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发表时间：
2005-08
期刊：
影响因子：
4.2
通讯作者：
Y. Sheng;W. Zheng;Ke-quan Pei;K. Ma
中科院分区：
文献类型：
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作者：
Y. Sheng;W. Zheng;Ke-quan Pei;K. Ma
背景与目的中国是受沙漠化影响最严重的国家之一。梭梭（Haloxylon ammodendron，苋科）是荒漠生态系统的重要生态组成部分，是荒漠生态系统修复的主要树种之一，但对其遗传结构了解甚少。方法利用ISSR （inter-simple sequence repeat）标记对中国两个地区9个梭梭居群内和居群间的遗传变异进行分析。8条引物扩增出219条可重复条带，其中184条（84%）为多态性条带。分子变异分析（AMOVA）显示群体内遗传变异高（97.63%），区域间和群体间遗传分化低（0.62%），群体间遗传分化低（1.75%）。结论目前的遗传结构可能是由高水平的基因流动引起的。在种群间观察到的基因流动可能主要归因于花粉运动。遗传结构在生态恢复实践中也具有重要意义。
BACKGROUND AND AIMS China is one of the countries most severely affected by desertification. Haloxylon ammodendron (Amaranthaceae) is an ecologically important component of the desert ecosystem and is one of the main tree species used for restoration, yet we know little about its genetic structure. METHODS Genetic variation within and between nine populations of H. ammodendron from two regions of China was investigated using ISSR (inter-simple sequence repeat) markers. KEY RESULTS Eight primers used in this study amplified 219 reproducible bands of which 184 (84 %) were polymorphic. Analysis of molecular variance (AMOVA) revealed high genetic variation within populations (97.63 %) and low genetic differentiation between regions (0.62 %) and among populations (1.75 %). CONCLUSIONS It is suggested that the present genetic structure could have arisen by high levels of gene flow. The gene flow among populations observed here is probably mainly attributable to pollen movement. The genetic structure also has important implications in ecological restoration practice.