The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae.
The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae.
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DOI：
10.1016/j.plaphy.2005.07.001
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发表时间：
2005-07
期刊：
影响因子：
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通讯作者：
Katja Stehfest;J. Toepel;C. Wilhelm
中科院分区：
文献类型：
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作者：
Katja Stehfest;J. Toepel;C. Wilhelm
利用微傅里叶变换红外光谱（FTIR）研究了3种蓝藻（铜绿微囊藻、分钟Croococcus和Nostoc sp.）和2种硅藻（meneghiniana Cyclotella和tricorodactylum）在营养胁迫下的光谱特征变化。根据生理关键参数和FTIR光谱法观察细胞饥饿4周后的生理状态变化。利用蛋白质、脂类、碳水化合物和硅酸盐的细胞光谱综合FTIR波段的变化来计算相对生物量组成。结果表明，短期驯化首先表现在色素沉着和光合效率上，而生物量组成的变化反映了代谢的长期调节。同时监测短期和长期的应激适应表明，应对日益增加的营养限制的代谢策略是高度物种特异性的。这种物种特异性只能通过单细胞技术在天然浮游植物样本中解决。结果表明，FTIR技术具有应用于天然浮游植物群落单细胞生物量组成测定的潜力。
Micro-Fourier transform infrared (FTIR) spectroscopy was used to study changes in spectral features of three species of Cyanobacteria (Microcystis aeruginosa, Croococcus minutus, and Nostoc sp.) and two Bacillariophyceae (Cyclotella meneghiniana, and Phaeodactylum tricornutum) in response to nutrient stress. The change of physiological state of the cells was followed during a 4-week starvation period on the basis of physiological key parameters and by means of FTIR spectroscopy. Changes in the integrated FTIR bands of cell spectra assigned to proteins, lipids, carbohydrates and silicate were used to calculate relative biomass composition. The results show that short-term acclimatization become visible at first in pigmentation and photosynthetic efficiency, whereas changes in biomass composition reflect long term modulation in the metabolism. Simultaneous monitoring of short term and long term stress acclimatization showed evidence that the metabolic strategies to cope with increasing nutrient limitation are highly species-specific. This species-specificity can only be resolved in natural phytoplankton samples by single cell techniques. The results show that the FTIR technique has the potential to become applicable for the determination of single cell biomass composition from natural phytoplankton communities.