A MODIFIED NINHYDRIN COLORIMETRIC ANALYSIS FOR AMINO ACIDS
A MODIFIED NINHYDRIN COLORIMETRIC ANALYSIS FOR AMINO ACIDS
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DOI：
10.1016/0003-9861(57)90241-2
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发表时间：
1957-01-01
影响因子：
3.9
通讯作者：
ROSEN, H
中科院分区：
文献类型：
--
作者：
ROSEN, H
本文介绍了一种测定氨基酸纯度的比色法。这是Yemm和Cocking方法的改进，使用氰化物和金刚三酮。本方法避免了制备难度大、储存不便的不稳定试剂--还三酮溶液的制备。该方法只用到两种试剂：纤维素三酮和乙酸酯氰化物。在分析前将它们分别加入含有氨基酸的溶液中；混合物在100摄氏度加热15分钟，然后稀释并冷却。用色度计测定得到的紫色溶液的光密度。该方法适用于氨基酸混合物，当考虑到颜色产率的微小变化和干扰化合物的存在时。
A colorimetric method for the quantitative analysis of pure amino acids is described. It is a modification of the method of Yemm and Cocking which employs cyanide and ninhydrin. The present method avoids the necessity for the preparation of solutions of reduced ninhydrin, which is an unstable reagent difficult to prepare and impracticable to store. Only 2 reagents are used in this method:cellosolve-ninhydrin and acetate-cyanide. They are added separately to the amino acid containing solution just before the analysis; the mixture is heated at 100[degree]C for 15 minutes, after which it is diluted and cooled. The optical density of the resulting purple solution is determined with a colorimeter. The method is applicable to amino acid mixtures when allowances are made for slight variability of color yields, and the presence of interfering compounds.