Bacteriophage cocktail significantly reduces or eliminates Listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods
Bacteriophage cocktail significantly reduces or eliminates Listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods
复制标题
DOI：
10.1016/j.fm.2015.06.006
复制
发表时间：
2015-12-01
影响因子：
5.3
通讯作者：
Sulakvelidze, Alexander
中科院分区：
文献类型：
--
作者：
Perera, Meenu N.;Abuladze, Tamar;Sulakvelidze, Alexander
ListShield(TM)是一种商用噬菌体鸡尾酒，专门针对单核细胞增生性李斯特菌，已被评估为各种即食食品中单核细胞增多性李斯特菌的生防剂。对实验污染的生菜、奶酪、熏鲑鱼和冷冻主菜进行ListShield T处理后，单核细胞增多性乳杆菌的污染率分别降低了91%(1.1log)、82%(0.7log)、90%(1.0log)和99%(2.2log)(p<；0.05)。单独应用ListShield T或与抗氧化剂/抗褐变液结合使用，在4℃下24小时后，苹果片上的单核细胞增多性李氏杆菌污染显著减少(p<；0.001)93%(1.1log)。对来自商业加工设施的熏鲑鱼进行ListShield T处理后，自然污染和实验污染的三文鱼鱼片中的单核细胞增多性李氏杆菌(无法检测到)。ListShield T的应用不会影响食品的感官质量，因为在颜色、味道或外观上没有检测到任何差异。利用ListShield T等裂解噬菌体制剂对单核细胞增多性李斯特菌进行生物控制，可以提供一种环境友好、绿色的方法，以减少与食用可能被单核细胞增多性李氏杆菌污染的各种食物相关的李斯特菌病的风险。(C)2015爱思唯尔有限公司。保留所有权利。
ListShield (TM), a commercially available bacteriophage cocktail that specifically targets Listeria monocytogenes, was evaluated as a bio-control agent for L. monocytogenes in various Ready-To-Eat foods. ListShield T treatment of experimentally contaminated lettuce, cheese, smoked salmon, and frozen entrees significantly reduced (p < 0.05) L. monocytogenes contamination by 91% (1.1 log), 82% (0.7 log), 90% (1.0 log), and 99% (2.2 log), respectively. ListShield T application, alone or combined with an antioxidant/anti-browning solution, resulted in a statistically significant (p < 0.001) 93% (1.1 log) reduction of L. monocytogenes contamination on apple slices after 24 h at 4 degrees C. Treatment of smoked salmon from a commercial processing facility with ListShield T eliminated L. monocytogenes (no detectable L. monocytogenes) in both the naturally contaminated and experimentally contaminated salmon fillets. The organoleptic quality of foods was not affected by application of ListShield T, as no differences in the color, taste, or appearance were detectable. Bio-control of L. monocytogenes with lytic bacteriophage preparations such as ListShield T can offer an environmentally-friendly, green approach for reducing the risk of listeriosis associated with the consumption of various foods that may be contaminated with L. monocytogenes. (C) 2015 Elsevier Ltd. All rights reserved.