Whey protein and alginate hydrogel microparticles for insulin intestinal absorption: Evaluation of permeability enhancement properties on Caco-2 cells
Whey protein and alginate hydrogel microparticles for insulin intestinal absorption: Evaluation of permeability enhancement properties on Caco-2 cells
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DOI：
10.1016/j.ijpharm.2013.06.016
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发表时间：
2013-09-10
影响因子：
5.8
通讯作者：
Beyssac, Eric
中科院分区：
文献类型：
--
作者：
Deat-Laine, Emmanuelle;Hoffart, Valerie;Beyssac, Eric
采用体外方法评价微囊化胰岛素的肠吸收促进作用。以乳清蛋白（WP）和海藻酸钠（ALG）为原料，采用冷凝胶法制备胰岛素微粒（INS-MP）。研究了INS包封对胰蛋白酶和糜蛋白酶降解的影响。使用体外（Caco-2细胞）实验进行渗透性研究。INS通过包封被部分保护以防止酶降解。此外，INS运输实验表明，WP和，在较小程度上，ALG能够提高INS吸收作为MP和聚合物溶液通过打开紧密连接。这些实验增强了在WP/ALG水凝胶组合中包封的兴趣。(C)2013爱思唯尔有限公司版权所有。
The evaluation of encapsulated insulin intestinal absorption enhancement was investigated by in vitro methods. Insulin-loaded microparticles (INS-MP) made of whey protein (WP) and alginate (ALG) were prepared by a cold gelation technique. Effect of INS encapsulation toward trypsin and chymotrypsin degradation was performed. Permeability studies using in vitro (Caco-2 cells) experiments were conducted. INS was partially protected by encapsulation toward enzymatic degradation. Moreover INS transport experiments showed that WP and, in lesser extent, ALG were able to enhance INS absorption both as MP and as polymeric solutions by opening the tight junctions. These experiments reinforced the interest of encapsulation in WP/ALG hydrogel combination. (C) 2013 Elsevier B.V. All rights reserved.