Ultrasensitive and facile detection of multiple trace antibiotics with magnetic nanoparticles and core-shell nanostar SERS nanotags
Ultrasensitive and facile detection of multiple trace antibiotics with magnetic nanoparticles and core-shell nanostar SERS nanotags
复制标题
使用磁性纳米粒子和核壳纳米星 SERS 纳米标签超灵敏、轻松地检测多种痕量抗生素
DOI：
10.1016/j.talanta.2021.122955
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发表时间：
2021-10-13
期刊：
影响因子：
6.1
通讯作者：
Zhao, Xiangwei
中科院分区：
文献类型：
--
作者：
Liu, Bing;Zheng, Shiya;Zhao, Xiangwei
超灵敏、多重、快速且准确地定量测定痕量抗生素仍然是一个具有挑战性的问题，这对公众健康和安全至关重要。在此，我们提出了一种基于磁性纳米粒子和表面增强拉曼散射（SERS）纳米标签的多重策略，用于同时检测氯霉素（CAP）和四环素（TTC）。在实际应用中，基于拉曼报告探针（RRPs）编码的金银核壳纳米星的SERS纳米标签被用作检测标记，以识别不同类型的抗生素，并且磁性纳米粒子可以通过磁力简单地分离，这显著提高了检测效率，降低了分析成本，并简化了操作。我们的结果表明，所提出的检测方法具有高灵敏度和多重检测能力，对CAP和TTC的检测限（LODs）分别为159.49和294.12 fg/mL，还具有良好的稳定性和重现性，以及高选择性和可靠性。我们相信，这种策略在微系统中检测痕量抗生素方面具有很大的应用前景，这对我们的生活至关重要。此外，通过改变合适的抗体或适体，该检测方法还可用于检测其他非法添加剂。
Ultrasensitive, multiplex, rapid, and accurate quantitative determination of trace antibiotics remains a challenging issue, which is of importance to public health and safety. Herein, we presented a multiplex strategy based on magnetic nanoparticles and surface-enhanced Raman scattering (SERS) nanotags for simultaneous detection of chloramphenicol (CAP) and tetracycline (TTC). In practice, SERS nanotags based on Raman reporter probes (RRPs) encoded gold-silver core-shell nanostars were used as detection labels for identifying different types of antibiotics, and the magnetic nanoparticles could be separated simply by magnetic force, which significantly improves the detection efficiency, reduces the analysis cost, and simplifies the operation. Our results demonstrate that the as-proposed assay possesses the capacities of high sensitivity and multiplexing with the limits of detection (LODs) for CAP and TTC of 159.49 and 294.12 fg mL(-1), respectively, as well as good stability and reproducibility, and high selectivity and reliability. We believe that this strategy holds a great promising perspective for the detection of trace amounts of antibiotics in microsystems, which is crucial to our life. Additionally, the assay can also be used to detect other illegal additives by altering the appropriate antibodies or aptamers.