A hormone and proteome approach to picturing the initial metabolic events during Plasmodiophora brassicae infection on Arabidopsis
A hormone and proteome approach to picturing the initial metabolic events during Plasmodiophora brassicae infection on Arabidopsis
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DOI：
10.1094/mpmi-19-1431
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发表时间：
2006-12-01
影响因子：
3.5
通讯作者：
Prinsen, Els
中科院分区：
文献类型：
--
作者：
Devos, Sylvie;Laukens, Kris;Prinsen, Els
本文报道了拟南芥在激素和蛋白质组水平上对专性生物营养型病原菌芸苔根肿菌（Plasmodiophora brassicae）的早期反应。使用CYCB 1; 1：：GUS构建体，从接种后4天开始显示与感染相关的细胞分裂的重新启动。增强的细胞分裂素基因的反应和积累的异戊烯基腺嘌呤和腺苷之前，这重新启动细胞分裂，而增强的生长素基因的反应，观察到从接种后6天on.alh1突变体，受损的乙烯和生长素之间的串扰，是耐P芸苔。感染与未感染的根和下胚轴的差异蛋白质分析进行了使用二维凝胶电泳和定量图像分析，再加上基质辅助激光解吸电离飞行时间-飞行时间质谱的蛋白质鉴定。在可视化的蛋白质中，与未感染的植物相比，12%的蛋白质显示出改变的丰度，包括参与代谢，细胞防御，细胞分化和解毒的蛋白质。结合激素和蛋白质组数据，我们假设，在疟原虫感染的第一阶段，疟原虫产生的细胞分裂素触发局部重新启动根皮层细胞分裂。因此，从头分生组织区域被建立，其充当宿主来源的吲哚-3-乙酸、碳水化合物、氮和能量的库，以维持病原体并触发虫瘿发育。
We report on the early response of Arabidopsis thaliana to the obligate biotrophic pathogen Plasmodiophora brassicae at the hormone and proteome level. Using a CYCB1;1::GUS construct, the re-initiation of infection-related cell division is shown from 4 days after inoculation on. Sensitivity to cytokinins and auxins as well as the endogenous hormone levels are evaluated. Both an enhanced cytokinin gene response and an accumulation of isopentenyl adenine and adenosine precede this re-initiation of cell division, whereas an enhanced auxin gene response is observed from 6 days after inoculation on. The alh1 mutant, impaired in the cross talk between ethylene and auxins, is resistant to P brassicae. A differential protein analysis of infected versus noninfected roots and hypocotyls was performed using two-dimensional gel ellectrophoresis and quantitative image analysis, coupled to matrix-assisted laser desorption ionization time of flight-time of flight mass spectrometry-based protein identification. Of the visualized proteins, 12% show altered abundance compared with the noninfected plants, including proteins involved in metabolism, cell defense, cell differentiation, and detoxification. Combining the hormone and proteome data, we postulate that, at the very first stages of Plasmodiophora infection, plasmodial-produced cytokinins trigger a local re-initiation of cell division in the root cortex. Consequently, a de novo meristematic area is established that acts as a sink for host-derived indole-3-acetic acid, carbohydrates, nitrogen, and energy to maintain the pathogen and to trigger gall development.