In vitro production of erythropoietin by kidneys perfused with a serum-free solution.
In vitro production of erythropoietin by kidneys perfused with a serum-free solution.
复制标题
通过用无血清溶液灌注的肾脏体外产生促红细胞生成素。
作者：
Allan J. Erslev
正常兔暴露于0.4大气压3小时将在随后的3小时期间产生约40-60 U的促红细胞生成素。如果肾脏从3小时缺氧的动物，仔细清洗，并通过再循环与血清组织培养混合物灌注3小时，每个肾脏产生约14 U的促红细胞生成素在体外。正常肾脏的灌注不会导致促红细胞生成素的产生，如果灌注液含有嘌呤霉素，则仅产生少量促红细胞生成素。发现用无血清组织培养基灌注3小时的低氧肾每个肾产生约8 U的促红细胞生成素，而用盐水灌注的类似肾约1U。这些结果表明，红细胞生成素是由肾组织合成的，而不是由血浆底物的酶促活化产生的。
Normal rabbits exposed to 0.4 atmospheric pressure for 3 hr will generate about 40-60 U of erythropoietin during a subsequent 3-hr period. If the kidneys were removed from 3-hr hypoxic animals, washed carefully, and perfused for 3 hr by recirculation with a serum-tissue culture mixture, each kidney generated about 14 U of erythropoietin in vitro. Perfusion of normal kidneys did not result in the production of erythropoietin, and only small amounts were generated if the perfusate contained Puromycin. Three-hour hypoxic kidneys perfused for 3 hr with a serum-free tissue culture medium were found to generate about 8 U of erythropoietin per kidney and similar kidneys perfused with saline about 1 U. These results indicate that erythropoietin is synthesized by kidney tissue and not produced by enzymatic activation of a plasma substrate.