Proteomic analysis of the entomopathogenic nematode Steinernema feltiae IS-6IJs under evaporative and osmotic stresses
Proteomic analysis of the entomopathogenic nematode Steinernema feltiae IS-6IJs under evaporative and osmotic stresses
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DOI：
10.1016/j.molbiopara.2005.10.003
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发表时间：
2006-02-01
影响因子：
1.5
通讯作者：
Wilson, MJ
中科院分区：
文献类型：
--
作者：
Chen, SB;Glazer, I;Wilson, MJ
为了提高广泛销售的基于昆虫病原线虫（EPN）的生物杀虫剂在干燥条件下的储存能力，我们需要了解这些生物对干燥胁迫的反应。作为我们的研究的一部分，以实现这一目标，我们研究了EPN斯氏线虫feltiae IS-6的感染性青少年暴露于蒸发（暴露于97%的相对湿度（RH）3天，随后1天暴露于85%RH）和渗透（暴露于24%甘油8小时）应力时的生存和蛋白质表达。通过蛋白质组学分析检测到的400多个蛋白质点在重复中显示出可重复的丰度。其中，10个点和7个点显示出可检测的变化，在蒸发和渗透胁迫下，分别与完全水合线虫的丰度。三个斑点在蒸发干燥和渗透干燥之间表现出不同的响应模式（一个在蒸发干燥中下调，两个在蒸发干燥中是新的）。MALDI-TOF质谱（MS）肽质量图谱鉴定了10个脱水反应蛋白，其中包括热休克蛋白60、辅酶q生物合成蛋白、肌醇单磷酸酶和延胡索酸裂解酶，它们在两种胁迫中都有发现。已知其他鉴定的蛋白质参与细胞周期调节、基因转录调节、大分子结构的组织，并且一些目前还没有已知的功能。我们的研究结果表明，这是不太可能的，可以通过遗传转化和添加单基因，选择性育种可能是最好的方法来产生抗干燥蠕虫的EPN的干燥耐受性的改善。(c)2005 Elsevier B. V.保留所有权利。
In order to improve the storage capability under desiccation of the widely sold biological insecticides based on entomopathogenic nematodes (EPNs), we need to understand how these organisms respond to desiccation stress. As part of our studies to achieve this, we studied survival and protein expression in infective juveniles of the EPN Steinernema feltiae IS-6 when exposed to evaporative (exposure to 97% relative humidity (RH) for 3 days, followed by a 1-day exposure to 85% RH) and osmotic (exposure to 24% glycerol for 8 h) stresses. More than 400 protein spots that were detected by proteomic analysis showed reproducible abundance within replications. Of these, 10 spots and 7 spots showed detectable changes in abundance under evaporative and osmotic stress, respectively, compared to fully hydrated nematodes. Three spots exhibited a differential response pattern between evaporative and osmotic desiccation (one was down regulated and two were novel in evaporative desiccation). Peptide mass mapping with MALDI-TOF mass spectrometry (MS) identified 10 desiccation-response proteins, among which several are known to be stress responsive including heat shock protein 60, coenzyme q biosynthesis protein, inositol monophosphatase and fumarate lyase that were found in both stresses. Other identified proteins are known to be involved in the cell cycle regulation, regulation of gene transcription, organization of macromolecular structure and some currently have no known functions. Our results suggest that it is unlikely that improvement of desiccation tolerance in EPNs can be achieved through genetic transformation and addition of single genes and that selective breeding could be the best approach to generate desiccation resistant worms. (c) 2005 Elsevier B.V. All rights reserved.