Precise relative ion abundances from Fourier transform ion cyclotron resonance magnitude-mode mass spectra.
Precise relative ion abundances from Fourier transform ion cyclotron resonance magnitude-mode mass spectra.
复制标题
来自傅里叶变换离子回旋共振震级模式质谱的精确相对离子丰度。
DOI：
10.1021/ac00200a013
复制
发表时间：
1990
影响因子：
7.4
通讯作者：
Marshall,AG
中科院分区：
文献类型：
--
作者：
Liang,ZM;Marshall,AG
在傅里叶变换光谱分析（离子回旋共振、磁共振、干涉吸收）中，正确定相的吸收模式光谱峰下的面积是振荡器（离子、自旋、分子）数量的直接量度。然而，相位校正可以证明是困难的，当（如在宽带傅里叶变换离子回旋共振（FT/ICR））检测是相当长的时间延迟后激发。在没有噪声的情况下，Huang、Rempel和Gross表明，对于非定相无噪声光谱，“复面积”方法产生正确的吸收模式峰面积。在本文中，我们表明，振荡器的数量也可以从一个最小二乘拟合的幅度模式（乐，相位无关）的频谱。理论推导和FT/ICR实验结果表明，在存在噪声和不存在峰重叠的情况下，幅值模方法的精度比基于幅值模峰高、“复面积”或甚至对正确定相的吸收模峰直接数字积分的方法的精度高上级。因此，本方法似乎提供了最好的可用的测定不同质荷比的离子的相对丰度的FT/ICR质谱。
The area under a correctly phased absorption-mode spectral peak Is a direct measure of the number of oscillators (Ions, spins, molecules) In Fourier transform spectrometry (Ion cyclotron resonance, magnetic resonance, Interferometry ab-sorbance). However, phase correction can prove difficult when (as In broad-band Fourier transform Ion cyclotron resonance (FT/ICR)) detection Is considerably time-delayed after excitation. In the absence of noise, Huang, Rempel, and Gross showed that a “complex area" method yields the correct absorption-mode peak area, for an unphased noiseless spectrum. In this paper, we show that the number of oscillators may also be obtained from a least-squares fit to a magnitude-mode (le, phase-independent) spectrum. In the presence of noise and In the absence of peak overlap, the magnitude-mode method offers precision superior to that based on magnitude-mode peak height,“complex area”, or even direct digital Integration of a correctly phased absorption-mode peak, as demonstrated by both theoretical derivation and experimental FT/ICR results. The present method thus appears to offer the best available determination of the relative abundances of Ions of different mass-to-charge ratio in FT/ICR mass spectrometry.