Induction of antiviral state in fish cells by Japanese flounder, Paralichthys olivaceus, interferon regulatory factor-1.
Induction of antiviral state in fish cells by Japanese flounder, Paralichthys olivaceus, interferon regulatory factor-1.
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DOI：
10.1016/j.fsi.2004.12.008
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发表时间：
2005-07
影响因子：
4.7
通讯作者：
C. Caipang;I. Hirono;T. Aoki
中科院分区：
文献类型：
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作者：
C. Caipang;I. Hirono;T. Aoki
干扰素调节因子-1（IRF-1）通过调节干扰素（IFN-α/β）系统的表达来介导细胞中的抗病毒状态。为了阐明IRF-1在鱼类病毒感染过程中的作用，我们构建了一个重组质粒的日本比目鱼，Paralichthys olivaceus IRF-1（JF IRF-1）的巨细胞病毒（CMV）立即/早期增强子启动子的控制下。利用同源细胞系的转染实验研究了JF IRF-1的抗病毒机制。在这里，我们表明，从瞬时转染的细胞获得的细胞上清液增强细胞活力的异源细胞系孵育后，降低滴度的Hirame弹状病毒（HIRRV）和病毒性出血性败血症病毒（VHSV），并具有类精氨酸的活性，所示的能力，以保护细胞免受病毒感染。上清液还在感染的早期阶段抑制弹状病毒的复制，如在从转染细胞获得的上清液存在下病毒滴度的降低所示。进一步的分析表明，细胞培养上清液含有具有酸不稳定性和耐温特性的类精氨酸物质。这些结果表明，JF IRF-1通过介导烟碱样物质的产生在细胞中诱导抗病毒状态。因此，JF IRF-1可能是一个有用的佐剂，在开发的DNA疫苗，对商业上重要的病毒病原体在日本比目鱼水产养殖。
Interferon regulatory factor-1 (IRF-1) mediates an antiviral state in cells by regulating the expression of the interferon (IFN-α/β) system. To elucidate the role of IRF-1 in fish during virus infections, we constructed a recombinant plasmid of the Japanese flounder, Paralichthys olivaceus IRF-1 (JF IRF-1) under the control of the cytomegalovirus (CMV) immediate/early enhancer promoter. The antiviral mechanism of JF IRF-1 was studied using transfection experiments in a homologous cell line. Here, we show that cell supernatants obtained from transiently transfected cells enhanced cell viability of a heterologous cell line upon incubation, reduced the titers of hirame rhabdovirus (HIRRV) and viral hemorrhagic septicemia virus (VHSV), and possessed cytokine-like activity, as shown by their ability to protect cells against virus infections. The supernatants also inhibited the replication of the rhabdoviruses during the early stages of infection as indicated by the reduction of viral titers in the presence of the supernatants obtained from the transfected cells. Further analysis showed that the cell culture supernatants contain cytokine-like substances that possess acid-labile and temperature-resistant properties. These results indicate that JF IRF-1 induces an antiviral state in cells by mediating the production of cytokine-like substances. Thus, JF IRF-1 might be useful as an adjuvant in the development of DNA vaccines against commercially important viral pathogens in Japanese flounder aquaculture.