Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula
Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula
复制标题
蒺藜苜蓿矮化基因Mini Plant 1 (MNP1)的克隆及功能分析
DOI：
10.3390/ijms21144968
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发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Chen, Jianghua
中科院分区：
文献类型：
--
作者：
Guo, Shiqi;Zhang, Xiaojia;Chen, Jianghua
株高是一个重要的农艺性状，由于株高与抗倒伏性之间的密切关系，在很大程度上决定了作物的产量。豆类在全球农业中发挥着独特的作用；然而，很少有人关注它们高度的分子基础。在这里，我们对模式豆科植物蒺藜苜蓿的第一个矮化突变体迷你植物 1 (mnp1) 进行了表征。我们的研究发现 mnp1 突变体的细胞长度和节间细胞数量均减少。利用正向遗传筛选和随后的全基因组重测序方法，我们克隆了 MNP1 基因，发现它编码与赤霉素 (GA) 生物合成第一步有关的推定柯巴基二磷酸合酶 (CPS)。 MNP1 与豌豆 LS 高度同源。亚细胞定位显示MNP1位于叶绿体中。进一步分析表明，GA3可以显着恢复mnp1-1的株高，并且MNP1在拟南芥cps1突变体中的表达部分挽救了其小植物表型，表明MNP1在GA生物合成中的保护功能。我们的结果为了解蒺藜草株高的遗传调控提供了有价值的信息。
Plant height is a vital agronomic trait that greatly determines crop yields because of the close relationship between plant height and lodging resistance. Legumes play a unique role in the worldwide agriculture; however, little attention has been given to the molecular basis of their height. Here, we characterized the first dwarf mutant mini plant 1 (mnp1) of the model legume plant Medicago truncatula. Our study found that both cell length and the cell number of internodes were reduced in a mnp1 mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned the MNP1 gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. MNP1 was highly homologous to Pisum sativum LS. The subcellular localization showed that MNP1 was located in the chloroplast. Further analysis indicated that GA3 could significantly restore the plant height of mnp1-1, and expression of MNP1 in a cps1 mutant of Arabidopsis partially rescued its mini-plant phenotype, indicating the conservation function of MNP1 in GA biosynthesis. Our results provide valuable information for understanding the genetic regulation of plant height in M. truncatula.