PACKAGING OF TRANSDUCING DNA BY BACTERIOPHAGE-P1
PACKAGING OF TRANSDUCING DNA BY BACTERIOPHAGE-P1
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DOI：
10.1007/bf00330490
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发表时间：
1988-11-01
期刊：
影响因子：
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通讯作者：
MASTERS, M
中科院分区：
文献类型：
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作者：
HANKS, MC;NEWMAN, B;MASTERS, M
P1以广泛不同的频率转导细菌染色体标记。我们在这里使用定量南方杂交来表明，尽管如此，大多数标记被包装在相似的水平。例外情况是在接近2分钟和另一个在90分钟的标记分组，似乎包装在两到三倍高的水平。因此，我们得出结论，某些标记频率的变化在转导可以解释包装水平的差异，但大多数不能。包装水平的有限范围表明P1可以从许多位点启动染色体DNA的包装。我们没有找到任何与噬菌体pac位点同源的染色体序列，并且杂交带的出现似乎表明来自大量特定位点的顺序包装，这一观点得到了支持。我们排除了染色体DNA包装是由P1酶进行核内溶解切割的结果的可能性。
P1 transduces bacterial chromosomal markers with widely differing frequencies. We use quantitative Southern hybridisations here to show that, despite this, most markers are packaged at similar level. Exceptions are grouped of markers near 2 min and another at 90 min which seem to be packaged at levels two- to threefold higher. We thus conclude that certain marker frequency variations in transduction can be explained by differences in packaging level, but that most cannot. The limited range in packaging levels suggests that P1 can initiate the packaging of chromosomal DNA from many sites. This idea is supported by our failure to find any chromosomal sequences with homology to the phage pac site and by the occurrence of hybridising bands which seem to suggest sequential packaging from a large number of specific sites. We eliminate the possibility that chromosomal DNA packaging is the result of endonucleolytic cutting by the P1 res enzyme.