Adipose tissue-derived stem cells attenuate acute lung injury through eNOS and eNOS-derived NO
Adipose tissue-derived stem cells attenuate acute lung injury through eNOS and eNOS-derived NO
复制标题
脂肪组织来源的干细胞通过 eNOS 和 eNOS 衍生的 NO 减轻急性肺损伤。
DOI：
10.3892/ijmm.2013.1328
复制
发表时间：
2013-06-01
影响因子：
5.4
通讯作者：
Wen, Qingping
中科院分区：
文献类型：
--
作者：
Gao, Peng;Yang, Xu;Wen, Qingping
急性肺损伤（ALI）是重症监护病房最常见的死亡原因之一。近年来的体内外研究表明，间充质干细胞（mesenchymal stem cells，MSCs）具有减轻肺水肿和炎症因子的作用，但MSCs对肺血管功能的影响机制尚不清楚。人们认为一氧化氮（NO）和内皮型一氧化氮合酶（eNOS）在调节血管功能和体内平衡中发挥着重要作用。在本研究中，我们研究了脂肪组织来源的干细胞（ADSCs）对肺微血管内皮细胞（PMVECs）和肺在脂多糖（LPS）诱导的ALI模型在体外和体内的影响。我们的研究结果表明，脂肪干细胞能够减轻ALI和肺水肿的严重程度。在肺PMVEC和用ADSC治疗后的肺中也观察到eNOS蛋白表达增加。此外，ADSC增加eNOS衍生的NO的浓度以重塑ALI。结果表明，脂肪干细胞可能是一个有前途的候选人，通过与eNOS和eNOS衍生的NO的相互作用，为ALI治疗。
Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Recent in vivo and in vitro studies have suggested that mesenchymal stem cells (MSCs) attenuate pulmonary edema and inflammatory factors, but the mechanisms of the effects of MSCs on pulmonary vascular function remain unknown. It is believed that nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) play an essential role in the regulation of vascular function and homeostasis. In the present study, we investigated the effect of adipose tissue-derived stem cells (ADSCs) on pulmonary microvascular endothelial cells (PMVECs) and the lung in a lipopolysaccharide (LPS)-induced ALI model in vitro and in vivo. Our results showed that ADSCs were able to attenuate the severity of ALI and pulmonary edema. Increased expression of the eNOS protein was also observed in pulmonary PMVECs and in the lung following treatment with ADSCs. Furthermore, ADSCs increased the concentration of eNOS-derived NO to remodel ALI. The results suggest that ADSCs may be a promising candidate for ALI treatment through interaction with eNOS and eNOS-derived NO.