Patterns of lambda Int recognition in the regions of strand exchange.
Patterns of lambda Int recognition in the regions of strand exchange.
复制标题
链交换区域的 lambda Int 识别模式。
DOI：
10.1016/0092-8674(83)90355-0
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发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
Landy,A
中科院分区：
文献类型：
--
作者：
Ross,W;Landy,A
Int蛋白在噬菌体尾部位点内具有两类结合位点：臂型识别序列存在于远离链交换区域的三个特定位点中;连接型识别序列在attf和attB中均以反向对的形式出现在交叉区域周围。在attf和attB之间的重组过程中，四条DNA链中的每一条都在每个连接型Int结合位点内的同源位置处被切割。在所有四个连接型位点中，Int蛋白主要与DNA螺旋的同一面相互作用，这取决于那些被硫酸二甲酯保护免受甲基化的嘌呤氮。X的有效二级附着位点包含与连接型结合位点部分同源的序列。此外，两个连接型位点（重叠区）之间的序列（但不是其一部分）在二级船尾位点中高度保守。因此，在链交换附近，attP和重组伴侣，如attB，是非常相似的;每个都包含两个junctiontype Int识别位点和一个重叠（交叉）区域。
Int protein has two classes of binding sites within the phage aft site: the arm-type recognition sequences are found in three specific sites that are distant from the region of strand exchange; the junction-type recognition sequences occur as inverted pairs around the crossover region in both attf and attB. During recombination between attf and attB each of the four DNA strands is cut at a homologous position within each of the junction-type Int binding sites. In all four junction-type sites Int protein interacts primarily with the same face of the DNA helix, as determined by those purine nitrogens that are protected against methylation by dimethylsulfate. Efficient secondary attachment sites for X contain sequences with partial homology to the junctiontype binding sites. In addition, the sequence between, but not part of, the two junction-type sites (the overlap region) is strongly conserved in secondary aft sites. Thus, in the vicinity of strand exchange, attP and a recombining partner, such as attB, are very similar; each comprises two junctiontype Int recognition sites and an overlap (crossover) region.