Museum specimens provide phylogenomic data to resolve relationships of sack‐bearer moths (Lepidoptera, Mimallonoidea, Mimallonidae)
Museum specimens provide phylogenomic data to resolve relationships of sack‐bearer moths (Lepidoptera, Mimallonoidea, Mimallonidae)
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DOI：
10.1111/syen.12301
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发表时间：
2018-05
影响因子：
4.8
通讯作者：
Ryan A. St Laurent;C. Hamilton;A. Kawahara
中科院分区：
文献类型：
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作者：
Ryan A. St Laurent;C. Hamilton;A. Kawahara
Mimallonidae是一种携带麻袋的飞蛾，是以新热带为主的一科，包含近300个已描述的物种。Mimallonidae以40多个寄主植物家族为食，并在各种环境中被发现，但该科内物种的系统发育关系从未被调查过。我们使用锚定杂交富集靶捕获对乙醇保存和干燥的博物馆标本中的515个基因座进行了测序，收集日期从1985年到2017年。我们采样了47个物种，代表了所描述的36个拟步甲属中的32个。通过纳入19个干燥的博物馆标本，并为每个标本恢复平均超过400个基因座，我们说明了自然历史收藏在锚定的基于浓缩的杂交系统发育学中的作用。最大似然法和多物种联合分析支持识别出Mimallonidae中的6个较高级别的类群，我们将它们指定为亚科：Zaphantinae St Laurent&Kawahara亚科、Aurorianinae St Laurent&Kawahara亚科、Mimallinae Burmeister、Lacosominae Dyar、Druentiinae St Laurent&Kawahara亚科n.和Cicinineae Schaus stat.n.我们的系统发育结果也有力地支持了8个新的部落：Lacosominae：Trogopterini St Laurent&Kawahara部落n.，Lacosomini Dyar stat.n.，Alheitini St Laurent&Kawahara部落n.；Druentiinae：Luramini St Laurent&Kawahara部落n.，Druentiini St Laurent&Kawahara部落n.；Cicinini亚科：Bedosiini St Laurent&Kawahara部落n.，Junchampini St Laurent&Kawahara部落n.，Cicinning Schastat.n.根据我们的系统发育结果，还描述了三个新属：Herbinalla St Laurent&Kawahara，GEN.N.，Ulaluma St Laurent&Kawahara，GEN.N.，Bedosiallo St Laurent&Kawahara，GEN.N.Naniteta Franclemont，syn.n.是Lacosoma Grote的同义词。新组合有6个：Macessoga laxa com.n.、Lacosoma elassa com.n.、Thaelia Anysia com.n.、Thaelia subrubiginosa com.n.、Herbinalla caudina com.n.、Druepabrosica com.n.、Ulala valva com.n.、Cicinnus egens com.n.、Roelmana plitidisata com.n.、Roelmana laguerrei com.n.、Junchocampa Joanna、Squelocampa unalca com.n.、eechocampa Hamudina com.n.、Junampa marona com.n.、Bedosiallo Eugenia com.n.、Bedosiallo eugenia com.n.、Roelmana mallforo bedosiallbebe.n.、Bedosiallo moengus com.n.、Bedosiallo Styx com.n.和Bedosiallo Sylvia com.n.这项研究是第一次在包括许多博物馆标本的全面分类数据集上实施LEP1探针集，我们的结果表明博物馆标本可以用于锚定杂交富集研究。重要的是，这些数据产生了一个强大的系统发育，将作为未来研究拟态植物进化的基础，如寄主植物关系和生物地理学。
Mimallonidae, the sack‐bearer moths, are a family of predominantly Neotropical moths containing nearly 300 described species. Mimallonidae feed on over 40 host plant families and are found in a variety of environments, but phylogenetic relationships of species within the family have never been investigated. We sequenced 515 loci using anchored hybrid enrichment target capture on ethanol‐preserved and dried museum specimens, with dates of collection ranging from 1985 to 2017. We sampled 47 species, representing 32 of the 36 described mimallonid genera. By incorporating 19 dry museum specimens, and recovering an average of over 400 loci for each, we illustrate the utility of natural history collections in anchored hybrid enrichment‐based phylogenomics. Maximum likelihood and multi‐species coalescent analyses provide robust support for the recognition of six higher‐level groups within Mimallonidae, which we designate as subfamilies: Zaphantinae St Laurent & Kawahara subfam.n., Aurorianinae St Laurent & Kawahara subfam.n., Mimalloninae Burmeister, Lacosominae Dyar, Druenticinae St Laurent & Kawahara subfam.n. and Cicinninae Schaus stat.n. Our phylogenetic results also robustly support eight new tribes: Lacosominae: Trogopterini St Laurent & Kawahara tribe n., Lacosomini Dyar stat.n., Alheitini St Laurent & Kawahara tribe n.; Druenticinae: Luramini St Laurent & Kawahara tribe n., Druenticini St Laurent & Kawahara tribe n.; Cicinninae: Bedosiini St Laurent & Kawahara tribe n., Psychocampini St Laurent & Kawahara tribe n., Cicinnini Schaus stat.n. Three new genera are also described based on our phylogenetic results: Herbinalla St Laurent & Kawahara, gen.n., Ulaluma St Laurent & Kawahara, gen.n., Bedosiallo St Laurent & Kawahara, gen.n. Naniteta Franclemont, syn.n. is a synonym of Lacosoma Grote. Six genera are paraphyletic, and in total 19 new combinations are proposed: Macessoga laxa comb.n., Lacosoma elassa comb.n., Thaelia anysia comb.n., Thaelia subrubiginosa comb.n., Herbinalla caudina comb.n., Druentica brosica comb.n., Ulaluma valva comb.n., Cicinnus eminens comb.n., Roelmana pluridiscata comb.n., Roelmana laguerrei comb.n., Psychocampa joanna comb.n., Psychocampa unalca comb.n., Psychocampa hamata comb.n., Psychocampa marona comb.n., Bedosiallo eugenia comb.n., Bedosiallo forbesi comb.n., Bedosiallo moengus comb.n., Bedosiallo styx comb.n. and Bedosiallo sylvia comb.n. This study is the first to implement the LEP1 probe set on a comprehensive taxonomic dataset that includes many museum specimens, and our results demonstrate that museum specimens can be used in anchored hybrid enrichment studies. Importantly, these data produce a robust phylogeny that will serve as a foundation for future studies on mimallonid evolution, such as host plant relationships and biogeography.