Transient-phase studies on the creatine kinase reaction. The analysis of a reaction pathway with three intermediates.
Transient-phase studies on the creatine kinase reaction. The analysis of a reaction pathway with three intermediates.
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肌酸激酶反应的瞬态研究。
DOI：
10.1111/j.1432-1033.1979.tb02043.x
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
R. Bertrand
中科院分区：
文献类型：
--
作者：
F. Travers;T. Barman;R. Bertrand
1.在毫秒范围内研究了肌酸激酶磷酸肌酸的初始形成，并研究了温度对瞬态和稳态阶段的影响。2.在25 ° C和35 ° C下，没有瞬态阶段。这与Gutfreund [Engelborghs，Y.，Marsh，A.，和Gutfreund，H.（1975）Biochem.J.151，47- 50]。3.在4摄氏度的磷酸肌酸形成的时间过程是复杂的，并包括三个短暂的阶段：滞后期，爆发期和稳态阶段。在此基础上提出了肌酸激酶的三个中间体的反应方案。尽管时间进程的完整性，提取估计的速率常数是困难的，计算机模拟和迭代的方法，不得不诉诸。4.试图为复合酶ADP、偏磷酸盐、肌酸对肌酸激酶反应途径的作用提供证据[参见Milner-White，E.J.和Watts，D.C.（1971）Biochem.J.122，727- 740]。在所使用的条件下，这些尝试在4摄氏度或35摄氏度下在低至2.5毫秒的时间内不成功。
1. The initial formation of creatine phosphate by creatine kinase was studied in the millisecond range and the effect of temperature on the transient and steady-state phases exploited. 2. At 25 degrees C and 35 degrees C there was no transient phase. This is in agreement with the results of Gutfreund [Engelborghs, Y., Marsh, A., and Gutfreund, H. (1975) Biochem. J. 151, 47--50]. 3. At 4 degrees C the time course of creatine phosphate formation was complex and consisted of three transient phases: a lag phase, a burst phase and a steady-state phase. Based on this result a reaction scheme for creatine kinase which includes three intermediates was proposed. Despite the completeness of the time course, the extraction of estimates for the rate constants was difficult and computer simulation and iterative methods had to be resorted to. 4. Attempts were made to provide evidence for the complex enzyme.ADP.metaphosphate.creatine on the creatine kinase reaction pathway [cf. Milner-White, E.J. and Watts, D.C. (1971) Biochem. J. 122, 727--740]. Under the conditions used these attempts were unsuccessful at times down to 2.5 ms, at 4 degrees C or 35 degrees C.