Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro
Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro
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DOI：
10.1530/rep.1.00735
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发表时间：
2005-10-01
期刊：
影响因子：
3.8
通讯作者：
Van Soom, A
中科院分区：
文献类型：
--
作者：
Leroy, JLMR;Vanholder, T;Van Soom, A
本研究测定了高产奶牛产后早期能量负平衡（NEB）期间卵泡液中非酯化脂肪酸（NEFA）的含量和组成。然后在体外成熟过程中以先前在FF中测量的浓度加入NEFA，以评估其对卵母细胞发育能力的影响。在产后16和44天，收集9头高产奶牛的优势卵泡FF和血液。分析样品的NEFA浓度和组成。NEB期间FF中的NEFA浓度（0.2- 0.6 mmol/l）与血清（0.4-1.2 mmol/l）相比仍低+/- 40%。血清和FF的NEFA组成存在显著差异，其中油酸（OA）、棕榈酸（PA）和硬脂酸（SA）是FF中的主要脂肪酸。基于这些结果，5115个卵母细胞在无血清培养基中成熟24小时，有或没有（阴性对照）添加溶解在乙醇中的0.200 mmol/l OA、0.133 mmol/l PA或0.067 mmol/l SA或仅乙醇（阳性对照）。将成熟的卵母细胞受精并在SOF培养基中培养7天。在卵母细胞成熟过程中添加PA或SA对卵母细胞的成熟、受精率、卵裂率和囊胚产量均有负面影响。在SA或PA存在下成熟的卵丘-卵母细胞复合体中观察到更多（晚期）凋亡的卵丘细胞。乙醇或OA没有影响。这些体外结果表明，NEB可能会阻碍生育力的高产奶牛通过增加NEFA浓度FF影响卵母细胞的质量。
in this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte's developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2-0.6mmol/l) during NEB remained +/- 40% lower compared with serum (0.4-1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus-oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality.