NUCLEOTIDE-SEQUENCE OF THE BROME MOSAIC-VIRUS GENOME AND ITS IMPLICATIONS FOR VIRAL REPLICATION
NUCLEOTIDE-SEQUENCE OF THE BROME MOSAIC-VIRUS GENOME AND ITS IMPLICATIONS FOR VIRAL REPLICATION
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DOI：
10.1016/s0022-2836(84)80012-1
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
KAESBERG, P
中科院分区：
文献类型：
--
作者：
AHLQUIST, P;DASGUPTA, R;KAESBERG, P
测定了雀麦花叶病毒(BMV)RNA1(3234个碱基)和2(2865个碱基)的核苷酸序列，完成了8200个碱基三部分BMV基因组的一级结构，获得了覆盖BMV RNA1 99%的c[互补]DNA克隆和BMV RNA2的全长cDNA克隆。广泛的序列同源性和已知与几种蛋白质的相互作用表明，BMV RNA的3‘’端是基因组的主要调控区。RNA1和RNA2 5‘端较小的区域彼此之间显示出很强的同源性，而与RNA3的同源性较低。这些序列和其他特征与BMV基因组的复制、调节和进化有关。
The nucleotide sequences of brome mosaic virus (BMV) RNA1 (3234 bases) and 2 (2865 bases) have been determined, completing the primary structure of the 8200 base tripartite BMV genome, c[complementary]DNA clones covering 99% of BMV RNA1 and a full-length cDNA clone of BMV RNA2 were isolated in the course of this work. Extensive sequence homology and known interaction with several proteins suggest that the 3'' ends of the BMV RNA are the major regulatory regions of the genome. Smaller regions at the 5'' ends of RNA1 and 2 show strong homology to each other and lesser homology to RNA3. These and other features of the sequences are discussed in relation to replication, regulation and evolution of the BMV genome.