Reproductive toxicity of UV-photodegraded polystyrene microplastics induced by DNA damage-dependent cell apoptosis in Caenorhabditis elegans
Reproductive toxicity of UV-photodegraded polystyrene microplastics induced by DNA damage-dependent cell apoptosis in Caenorhabditis elegans
复制标题
紫外线光降解聚苯乙烯微塑料诱导秀丽隐杆线虫 DNA 损伤依赖性细胞凋亡的生殖毒性
DOI：
10.1016/j.scitotenv.2021.152350
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发表时间：
2021-12-18
影响因子：
9.8
通讯作者：
Yu, Yunjiang
中科院分区：
文献类型：
--
作者：
Chen, Haibo;Yang, Yue;Yu, Yunjiang
虽然许多研究已经研究了聚苯乙烯微塑料(PS-MPS)的毒性效应，但自然老化的PS-MPS对土壤生物的毒性仍不清楚。研究了紫外光照射下原始PS-MPS的光降解行为，并检测了环境浓度(0.1~100mug/L)的原始PS-MPS和紫外光降解PS-MPS对秀丽线虫的生殖毒性。以孵化率和排卵率为终点，急性暴露于老化PS-MPS的生殖毒性比原始PS-MPS更严重。暴露于100ug/L老化的PS-MPS后，HUS-1：：GFP灶数目和DNA损伤所需基因CLK-2、CEP-1和EGL-1的表达显著增加，表明PS-MPS具有诱导DNA损伤的作用。此外，细胞身体数量和与凋亡相关的基因(如Ced-3、Ced-4和Ced-9)的表达也发生了显著变化，表明诱导了细胞凋亡。在EGL-1、hus-1、cep-1、ce-3、ce-4和ce-9突变体中，老化的PS-MPS诱导的生殖系细胞凋亡发生了改变。因此，老化PS-MPS的生殖毒性可能与DNA损伤诱导细胞凋亡有关，而HUS-1CEP-1-EGL-1-CED-9-CED-4-CED-3信号通路参与了线虫细胞凋亡的调控。
Although many studies have investigated the toxic effects of polystyrene microplastics (PS-MPs), toxicity of natural aged of PS-MPs to soil organisms remains unclear. The photodegradation of virgin PS-MPs under UV irradiation was investigated, and reproductive toxicity of pristine and UV-photodegraded PS-MPs at environmental concentrations (0.1-100 mu g/L) was examined to Caenorhabditis elegans. Using brood size and egg ejection rate as endpoints, acute exposure to aged PS-MPs resulted in more severe reproductive toxicity than pristine PS-MPs. Exposure to 100 mu g/L aged PS-MPs significantly increased the number of HUS-1::GFP foci and the expression of genes required for DNA damage, such as clk-2, cep-1, and egl-1, suggesting induction of DNA damage. Additionally, the number of cell corpses and apoptosis-related gene expression (e.g., ced-3, ced-4, and ced-9) were significantly altered, indicating induction of apoptosis. Germline apoptosis induced by aged PS-MPs was altered in egl-1, hus-1, cep-1, ced-3, ced-4, and ced-9 mutants. Thus, the reproductive toxicity of aged PS-MPs may be due to DNA damage-induced cell apoptosis, and the HUS-1CEP-1-EGL-1-CED-9-CED-4-CED-3 signalling pathway is involved in regulating cell apoptosis in nematodes.