The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
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DOI：
10.1105/tpc.106.049981
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发表时间：
2007-09-01
期刊：
影响因子：
11.6
通讯作者：
Chua, Nam-Hai
中科院分区：
文献类型：
--
作者：
Catala, Rafael;Ouyang, Jian;Chua, Nam-Hai
小泛素样修饰物（SUMO）对蛋白质的翻译后修饰调节蛋白质的降解和定位、蛋白质-蛋白质相互作用和转录活性。SUMO E3连接酶功能由酵母中的SIZ 1/SIZ 2和Mms 21、皮亚斯家族成员RanBP 2和人类中的Pc 2执行。拟南芥基因组仅包含一个基因SIZ 1，其与酵母SIZ 1/SIZ 2直向同源。在这里，我们表明，拟南芥SIZ 1在所有植物组织中表达。与野生型相比，无效突变体siz 1 -3的身材较小，因为参与油菜素类固醇生物合成和信号传导的基因表达减少。干旱胁迫诱导SUMO-蛋白缀合物的积累，这部分依赖于SIZ 1，但不依赖于脱落酸（阿坝）。大小1 -3的突变体对干旱胁迫的耐受性显著降低。一个全基因组表达分析确定了1700个拟南芥基因，干旱诱导，SIZ 1介导的DREB 2A和阿坝的独立途径的表达，其中300个。SIZ 1依赖的干旱响应基因包括那些编码花青素合成途径和茉莉酸响应酶的基因。从这些结果中，我们得出结论，SIZ 1调节拟南芥的生长和SUMO E3连接酶在干旱胁迫反应中发挥作用，可能通过调节基因表达。
Posttranslational modifications of proteins by small ubiquitin-like modifiers (SUMOs) regulate protein degradation and localization, protein-protein interaction, and transcriptional activity. SUMO E3 ligase functions are executed by SIZ1/SIZ2 and Mms21 in yeast, the PIAS family members RanBP2, and Pc2 in human. The Arabidopsis thaliana genome contains only one gene, SIZ1, that is orthologous to the yeast SIZ1/SIZ2. Here, we show that Arabidopsis SIZ1 is expressed in all plant tissues. Compared with the wild type, the null mutant siz1-3 is smaller in stature because of reduced expression of genes involved in brassinosteroid biosynthesis and signaling. Drought stress induces the accumulation of SUMO-protein conjugates, which is in part dependent on SIZ1 but not on abscisic acid (ABA). Mutant plants of siz1-3 have significantly lower tolerance to drought stress. A genome-wide expression analysis identified; 1700 Arabidopsis genes that are induced by drought, with SIZ1 mediating the expression of 300 of them by a pathway independent of DREB2A and ABA. SIZ1-dependent, drought-responsive genes include those encoding enzymes of the anthocyanin synthesis pathway and jasmonate response. From these results, we conclude that SIZ1 regulates Arabidopsis growth and that this SUMO E3 ligase plays a role in drought stress response likely through the regulation of gene expression.