T-CELL STIMULATION VIA THE ERYTHROCYTE RECEPTOR - SYNERGISM BETWEEN MONOCLONAL-ANTIBODIES AND PHORBOL-MYRISTATE ACETATE WITHOUT CHANGES OF FREE CYTOPLASMIC CA++ LEVELS
T-CELL STIMULATION VIA THE ERYTHROCYTE RECEPTOR - SYNERGISM BETWEEN MONOCLONAL-ANTIBODIES AND PHORBOL-MYRISTATE ACETATE WITHOUT CHANGES OF FREE CYTOPLASMIC CA++ LEVELS
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DOI：
10.1084/jem.163.3.654
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发表时间：
1986-03-01
影响因子：
15.3
通讯作者：
KNAPP, W
中科院分区：
文献类型：
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作者：
HOLTER, W;FISCHER, GF;KNAPP, W
我们观察到，某些E受体抗体(CD2抗体)可以在PMA存在的情况下诱导静息T细胞的增殖，而其他CD2抗体则没有这种作用。同样的CD2抗体在PMA(9.6，X11，VIT13)的存在下是有丝分裂的，但不是无反应的，它们也能够通过所谓的替代T细胞激活途径诱导T细胞增殖，即当在没有PMA的情况下以特定的组合与T细胞配对时。同时加入两种共基因CD2抗体(9.6或X11+VIT13)或单独加入一种非丝裂原CD3抗体(VIT3)可使细胞内钙离子水平明显升高，而单独加入一种CD2抗体则无此作用。即使在PMA存在的情况下，一个共基因CD2抗体单独也不能触发显著的钙反应，尽管这种结合诱导了增殖反应。这些数据表明，通过CD2激活T细胞有两种不同的机制，这与它们对胞浆游离钙离子的影响没有区别。当两种抗体同时触发时，细胞增殖先于钙离子水平的增加，而一种抗体加PMA的刺激导致细胞增殖，而没有可测量的早期钙反应。我们的结论是，单独用某些CD2抗体处理的T细胞已经识别了一个可能与钙稳态无关的激活信号，这个信号可以由PMA进一步发展，以重新启动完全发展的增殖反应。
We observed that certain E-receptor antibodies (CD2 antibodies) can induce proliferation of resting human T cells in the presence of PMA, while other CD2 antiodies fail to have such an effect. The same CD2 antibodies that were mitogenic in the presence of PMA (9.6, X11, VIT13), but not the nonreactive ones, were also able to induce T cell proliferation via the so-called alternative pathway of T cell activation, i.e., when added pairwise in certain combinations to T cells in the absence of PMA. While the simultaneous addition of two comitogenic CD2 antibodies (9.6 or X11 plus VIT13) or the addition of a single nonmitogenic CD3 antibody (VIT3) led to a clearcut elevation of intracellular Ca2+ levels, no such effect could be observed after the addition of one CD2 antibody alone. Even in the presence of PMA, one comitogenic CD2 antibody alone was unable to trigger a significant Ca2+ response, although this combinaton induced a proliferative response. These data indicate that, indistinguishable by their influence on free cytoplasmic Ca2+, there are two different mechanisms of T cell activation via CD2. While simultaneous triggering with two antibodies leads to cell proliferation preceded by an increase of Ca2+ levels, stimulation with one antibody plus PMA results in proliferaton without a measurable early Ca2+ response. We conclude that T cells treated by certain CD2 antibodies alone already recognize an activation signal probably unrelated to Ca2+ homeostasis, a signal that can further be developed by PMA to reuslt in a completely developed proliferative response.