Formation of precise connections in the olfactory bulb occurs in the absence of odorant-evoked neuronal activity
Formation of precise connections in the olfactory bulb occurs in the absence of odorant-evoked neuronal activity
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DOI：
10.1016/s0896-6273(00)81139-3
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发表时间：
2000-04-01
期刊：
影响因子：
16.2
通讯作者：
Brunet, L
中科院分区：
文献类型：
--
作者：
Lin, DM;Wang, F;Brunet, L
表达相同气味感受器的嗅觉神经元在嗅球内汇聚成少量的肾小球。反过来，二尖瓣和簇状细胞接收这些信息，并将其传递到更高级的皮质区域。在其他感觉系统中，相关的神经元活动被认为在发育过程中完善突触连接。我们询问，在缺乏功能性嗅觉环核苷酸门控(CNG)通道的小鼠中，当气味诱发信号被消除时，嗅觉感觉轴突和二尖瓣细胞树突之间的连接模式是否受到影响。我们证明了嗅觉轴突在CNG通道突变背景下正常会聚。我们进一步证明，二尖瓣细胞树突的修剪，尽管在发育过程中变慢，但在突变动物中最终是不受干扰的。因此，嗅觉CNG通道-由此推断相关的神经活动-不是在嗅球中产生突触特异性所必需的。
Olfactory neurons expressing the-same odorant receptor converge to a small number of glomeruli in the olfactory bulb. In turn, mitral and tufted cells receive and relay this information to higher cortical regions. In other sensory systems, correlated neuronal activity is thought to refine synaptic connections during development. We asked whether the pattern of connections between olfactory sensory axons and mitral cell dendrites is affected when odor-evoked signaling is eliminated in mice lacking functional olfactory cyclic nucleotide-gated (CNG) channels. We demonstrate that olfactory sensory axons converge normally in the CNG channel mutant background. We further show that the pruning of mitral cell dendrites, although slowed during development, is ultimately unperturbed in mutant animals. Thus, the olfactory CNG channel-and by inference correlated neural activity-is not required for generating synaptic specificity in the olfactory bulb.