Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration.
Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration.
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
W. Forrester;Elliot A. Perens;J. Zallen;G. Garriga
中科院分区：
文献类型：
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作者：
W. Forrester;Elliot A. Perens;J. Zallen;G. Garriga
为了了解引导细胞迁移的机制，我们一直在研究秀丽隐杆线虫（C. elegans）的管道相关神经元（can）的胚胎迁移。在这里，我们描述了用于鉴定参与CAN迁移的基因的两种筛选。首先，我们筛选了作为清晰幼虫（Clr）或有枯尾（Wit）死亡的突变体，这些表型在缺乏正常CAN功能的动物中表现出来。其次，我们直接筛选缺失或错位的can突变体。我们分离并鉴定了30个突变体，这些突变体定义了CAN迁移所需的14个基因。我们发现其中一个基因ceh-10决定了CAN的命运。ceh-10在分子上被定义为编码在can中表达的同源结构域蛋白。减少ceh-10功能的突变导致患有can的Wit动物在迁移过程中出现部分缺陷。消除ceh-10功能的突变导致具有CAN的Clr动物无法迁移或表达CEH-23 （CAN分化标记物）。空突变体也不能表达CEH-10，这表明CEH-10调节其自身的表达。最后，我们发现ceh-10对于另外两个表达ceh-10的细胞AIY和RMED的分化是必需的。
To understand the mechanisms that guide migrating cells, we have been studying the embryonic migrations of the C. elegans canal-associated neurons (CANs). Here, we describe two screens used to identify genes involved in CAN migration. First, we screened for mutants that died as clear larvae (Clr) or had withered tails (Wit), phenotypes displayed by animals lacking normal CAN function. Second, we screened directly for mutants with missing or misplaced CANs. We isolated and characterized 30 mutants that defined 14 genes necessary for CAN migration. We found that one of the genes, ceh-10, specifies CAN fate. ceh-10 had been defined molecularly as encoding a homeodomain protein expressed in the CANs. Mutations that reduce ceh-10 function result in Wit animals with CANs that are partially defective in their migrations. Mutations that eliminate ceh-10 function result in Clr animals with CANs that fail to migrate or express CEH-23, a CAN differentiation marker. Null mutants also fail to express CEH-10, suggesting that CEH-10 regulates its own expression. Finally, we found that ceh-10 is necessary for the differentiation of AIY and RMED, two additional cells that express CEH-10.