Ethylene Inhibits Abscisic Acid-Induced Stomatal Closure in Arabidopsis1
Ethylene Inhibits Abscisic Acid-Induced Stomatal Closure in Arabidopsis1
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DOI：
10.1104/pp.105.063503
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发表时间：
2005-07
期刊：
影响因子：
7.4
通讯作者：
Yoko Tanaka;T. Sano;M. Tamaoki;N. Nakajima;N. Kondo;S. Hasezawa
中科院分区：
文献类型：
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作者：
Yoko Tanaka;T. Sano;M. Tamaoki;N. Nakajima;N. Kondo;S. Hasezawa
为了研究脱落酸（ABA）和乙烯信号转导通路之间的串扰，ABA诱导的气孔关闭过程中的信号事件进行了研究，在拟南芥（拟南芥）野生型植物，在乙烯过量产生的突变体（eto1 - 1），并在两个乙烯不敏感的突变体（etr1 - 1和ein3 - 1）。使用孤立的表皮，气孔的野生型植物被发现在几分钟内关闭响应ABA，而气孔的eto1 - 1突变体表现出类似的，但不太敏感的ABA响应。此外，ABA诱导的气孔关闭可被乙烯或乙烯前体1-氨基环丙烷-1-羧酸（ACC）抑制。与此相反，气孔的etr1 - 1和ein3 - 1突变体能够关闭响应伴随ABA和ACC的应用程序，虽然在较小的程度上比野生型植物。此外，ABA诱导的基因RAB 18的表达在ACC应用后降低。这些结果表明，乙烯通过抑制ABA信号通路延迟气孔关闭。乙烯对ABA灌溉植株和干旱条件下植株气孔关闭的抑制作用相同。此外，在干旱胁迫下，蒸腾速率在eto1 - 1和野生型植物暴露于乙烯比未处理的野生型对照植物，表明乙烯对ABA诱导的气孔关闭的抑制作用也观察到在植物。
To examine the cross talk between the abscisic acid (ABA) and ethylene signal transduction pathways, signaling events during ABA-induced stomatal closure were examined in Arabidopsis (Arabidopsis thaliana) wild-type plants, in an ethylene-overproducing mutant (eto1-1), and in two ethylene-insensitive mutants (etr1-1 and ein3-1). Using isolated epidermal peels, stomata of wild-type plants were found to close within a few minutes in response to ABA, whereas stomata of the eto1-1 mutant showed a similar but less sensitive ABA response. In addition, ABA-induced stomatal closure could be inhibited by application of ethylene or the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). In contrast, stomata of the etr1-1 and ein3-1 mutants were able to close in response to concomitant ABA and ACC application, although to a lesser extent than in wild-type plants. Moreover, expression of the ABA-induced gene RAB18 was reduced following ACC application. These results indicate that ethylene delays stomatal closure by inhibiting the ABA signaling pathway. The same inhibitive effects of ethylene on stomatal closure were observed in ABA-irrigated plants and the plants in drought condition. Furthermore, upon drought stress, the rate of transpiration was greater in eto1-1 and wild-type plants exposed to ethylene than in untreated wild-type control plants, indicating that the inhibitive effects of ethylene on ABA-induced stomatal closure were also observed in planta.