Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L.
Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L.
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DOI：
10.1093/pcp/pch118
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发表时间：
2004-08-01
影响因子：
4.9
通讯作者：
Yamagushi-Shinozaki, KY
中科院分区：
文献类型：
--
作者：
Qin, F;Sakuma, Y;Yamagushi-Shinozaki, KY
转录因子DREB 1 s/CBFs与DRE/CRT顺式作用元件（核心基序：G/ACCGAC）特异性相互作用，控制拟南芥中许多胁迫诱导基因的表达。我们使用酵母单杂交系统在玉米中分离了DREB 1/CBF同源物ZmDREB 1A的cDNA。ZmDREB 1A蛋白与DRE特异性结合，ERF/AP 2 DNA结合域第14位高度保守的缬氨酸是决定该蛋白与DRE序列特异性相互作用的关键。ZmDREB 1A的表达受冷胁迫诱导，高盐胁迫略有增加。该基因也通过机械攻击瞬时表达。ZmDREB 1A在水稻原生质体中激活DRE驱动的GUS报告基因的转录。ZmDREB 1A在转基因拟南芥中的过表达诱导拟南芥DREB 1A的目标胁迫诱导基因的过表达，从而导致植物对干旱和冷冻胁迫具有更高的耐受性。这表明ZmDREB 1A与拟南芥DREB 1 s/CBFs具有相似的功能。玉米ZmDREB 1A的ERF/AP 2结构域与单子叶植物DREB 1型ERF/AP 2结构域的结构密切相关。ZmDREB 1A被认为是潜在的有用的生产转基因植物，是耐受干旱，高盐和/或冷胁迫。
The transcription factors DREB1s/CBFs specifically interact with the DRE/CRT cis-acting element (core motif: G/ACCGAC) and control the expression of many stress-inducible genes in Arabidopsis. We isolated a cDNA for a DREB1/CBF homolog, ZmDREB1A in maize using a yeast one-hybrid system. The ZmDREB1A proteins specifically bound to DRE and the highly conserved valine at the 14th residue in the ERF/AP2 DNA binding domain was a key to determining the specific interaction between this protein and the DRE sequence. Expression of ZmDREB1A was induced by cold stress and slightly increased by high-salinity stress. This gene was also transiently expressed by mechanical attack. ZmDREB1A activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Overexpression of ZmDREB1A in transgenic Arabidopsis induced overexpression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought and freezing stresses. This indicated that ZmDREB1A has functional similarity to DREB1s/CBFs in Arabidopsis. The structure of the ERF/AP2 domain of ZmDREB1A in maize is closely related to DREB1-type ERF/AP2 domains in the monocots as compared with that in the dicots. ZmDREB1A is suggested to be potentially useful for producing transgenic plants that is tolerant to drought, high-salinity and/or cold stresses.