Use of strontium for bovine oocyte activation
Use of strontium for bovine oocyte activation
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DOI：
10.1016/j.theriogenology.2004.08.012
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发表时间：
2005-05-01
期刊：
影响因子：
2.8
通讯作者：
Garcia, JM
中科院分区：
文献类型：
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作者：
Méo, SC;Yamazaki, W;Garcia, JM
锶可以有效地激活小鼠卵母细胞，然而，关于锶在牛身上的应用信息有限。因此，本研究的目的是建立一种合适的锶激活牛卵母细胞的方案。对于原核发育，10和50 mm锶的活化液(TALP)中不含钙和镁(分别为34.4%和53.1%)优于完全活化液(分别为6.5%和19.4%)。在所有的活化介质中，以25和50 mM锶的活化效果较好(分别为21.9-53.1%和19.4-53.1%)。孵育4h后，所有浓度的锶都有相似的结果。然而，15、20和25 mm的锶作用6和8h(分别为40.7、46.7和48.3%，29.3、48.3和40.7%)优于对照组(分别为15.5%和10%)。体外成熟26h后，锶(S；20 mM无钙镁离子滑石粉处理6h)、离子霉素+锶(IS)和锶+离子霉素(SI)的原核发育相似(分别为60%、63.3%和65%)，优于离子霉素(I；5 mM×5分钟；36.7%)。在S和处理I中，仅观察到1个PN合子。S组以1、2PB居多(35.7%、60.7%)，I组多为0、1、2PB(14.3%、57.1%、28.6%)。处理IS和SI的受精卵大多为1PN2PB(分别为77.4%和61.6%)。所有处理组的卵母细胞带有皮质颗粒簇的数量相似(11-29%)。治疗组皮质颗粒吐出率(68%)与S组(54%)相似，优于1、SI和对照组(分别为27%、45%和5.0%)。卵裂率和囊胚率在S组、I组、IS组和SI组相似(分别为61.7%-76.7%和8.3%-13.3%)，ICM、TE、细胞总数和ICM/总细胞比率也相同(22-25，-69和86-95，0.26-0.27)。总之，锶可以有效地应用于牛卵母细胞在20 mm无钙镁离子的TALP介质中激活6小时。(C)2004 Elsevier Inc.保留所有权利。
Strontium efficiently activates mouse oocytes, however, there is limited information on its use in cattle. Thus, the objective of this study was to establish a suitable protocol for activating bovine oocyte with strontium. For pronuclear development, the absence of calcium and magnesium in the activation medium (TALP) with 10 and 50mM strontium (34.4 and 53.1%, respectively) was superior to the complete TALP (6.5 and 19.4%, respectively). In all activation media, better results were observed with 25 and 50 mM strontium (21.9-53.1 and 19.4-53.1%, respectively). Incubation for 4 h promoted similar results in all strontium concentrations. However, strontium at 15, 20, and 25 mM for 6 and 8 h (40.7, 46.7, and 48.3%, and 29.3, 48.3, and 40.7%, respectively) were superior to control (15.5 and 10%, respectively). After in vitro maturation for 26 h, strontium (S; 20 mM in Ca2+ and Mg2+-free TALP for 6 h), ionomycin + strontium (IS), and strontium + ionomycin (SI) (60, 63.3, and 65%, respectively) were similar in pronuclear development and superior to ionomycin (I; 5 mu M for 5 min; 36.7%). In treatments S and I, only 1 PN zygotes were observed. In treatment S, most of them had 1 and 2 PB (35.7 and 60.7%, respectively), and in treatment I, 0, 1, and 2 PB (14.3, 57.1, and 28.6%, respectively). Most of the zygotes in treatment IS and SI were 1 PN 2 PB (77.4 and 61.6%, respectively). The number of oocytes with clusters of cortical granules was similar in all treated groups (11-29%). Cortical granule exocytosis in treatment IS (68%) was similar to S (54%) and superior to 1, SI, and control (27, 45, and 5.0%, respectively). Cleavage and blastocyst rates were similar for S, I, IS, and SI treatments (61.7-76.7, and 8.3-13.3%, respectively) and the same was observed for ICM, TE, and total cell number, and ICM/total cell ratio (22-25, 64-69, and 86-95, and 0.26-0.27). In conclusion, strontium may be efficiently applied for bovine oocyte activation at 20 mM in Ca2+-and Mg2+-free TALP medium for 6 h. (c) 2004 Elsevier Inc. All rights reserved.