Comparative proteomic analysis on acquired enamel pellicle at two time points in caries-susceptible and caries-free subjects
Comparative proteomic analysis on acquired enamel pellicle at two time points in caries-susceptible and caries-free subjects
复制标题
易患龋和无龋受试者两个时间点后天牙釉质的比较蛋白质组学分析
DOI：
10.1016/j.jdent.2020.103301
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发表时间：
2020-03-01
影响因子：
4.4
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
--
作者：
Luo, Junyuan;Wang, Yufei;Zhang, Linglin
目的：利用无标记和平行反应监测(PRM)蛋白质组学方法，分析易龋者和无龋者在两个时间点(5min和2 h)获得的釉质膜(AEP)的蛋白质谱，寻找潜在的龋病生物标志物。分别于5min和2 h取材，用非标记蛋白质组学方法对AEP材料进行定量分析。结果：高龋组和无龋组在5分钟和2小时点分别鉴定出82个和39个差异表达蛋白质。鉴定了一些与免疫应答和抗菌活性相关的蛋白质，并利用生物信息学分析给出了上调和下调蛋白的特征丰富的生物学过程。随后，从每组中选择了5个有趣的目标蛋白进行PRM分析，并分别在5分钟和2小时的AEP中成功地验证了它们的存在。结论：在这两个时间点，易龋者和无龋者的AEP中的蛋白质具有独特的与免疫反应和抗菌活性相关的蛋白质谱。粘蛋白-7可作为一种潜在的龋病生物标志物。临床意义：本研究结果确定了潜在的生物标志物，有助于设计新的生物功能制剂用于龋病的诊断和治疗。
Objectives: The aim of this study was to analyse the protein profiles of acquired enamel pellicle (AEP) at two time points (5 min and 2 h) between caries-susceptible and caries-free subjects using label-free and parallel reaction monitoring (PRM) proteomic approaches, and to discover potential biomarkers for dental caries.Methods: Sixty participants (30 caries-susceptible (DMFT > 5) and 30 caries-free subjects (DMFT = 0)) were included. Their AEP at 5 min and 2 h was separately sampled, and the AEP materials were quantitatively analysed using label-free proteomics. Bioinformatics analysis of differentially expressed proteins was subsequently conducted and target proteins were verified using PRM analysis.Results: A total of 82 and 39 differentially expressed proteins were identified at the 5-minute and 2-h points, respectively, between the caries-susceptible and caries-free groups. Some proteins related to immune response and antibacterial activity were identified, and the characteristic enriched biological processes of up- and down-regulated proteins were presented using bioinformatics analysis. Subsequently, five intriguing target proteins of each group were chosen for PRM analysis and their presence was successfully verified in the 5-minute and 2-h AEPs, respectively.Conclusions: The proteins in the AEPs of caries-susceptible and caries-free subjects at both time points presented unique protein profiles related to immune response and antibacterial activity. Mucin-7 can be regarded as an important potential AEP biomarker for dental caries.Clinical significance: The results of the study identified potential biomarkers, which can facilitate the design of new bio-functional agents for the diagnosis and treatment of dental caries in future.