DEVELOPMENT OF LEISHMANIA-MAJOR IN PHLEBOTOMUS-DUBOSCQI AND SERGENTOMYIA-SCHWETZI (DIPTERA, PSYCHODIDAE)
DEVELOPMENT OF LEISHMANIA-MAJOR IN PHLEBOTOMUS-DUBOSCQI AND SERGENTOMYIA-SCHWETZI (DIPTERA, PSYCHODIDAE)
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DOI：
10.4269/ajtmh.1990.43.31
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发表时间：
1990-07-01
影响因子：
3.3
通讯作者：
ROBERTS, CR
中科院分区：
文献类型：
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作者：
LAWYER, PG;NGUMBI, PM;ROBERTS, CR
在2种咬人的沙蝇中观察到了硕大利什曼原虫的外部发育，即已知的媒介杜氏白蛉和假定的非媒介施韦茨塞金蝇。在0、6、12、18、24、36、48和60小时和4时检查以仓鼠鼻部利什曼病损为食的苍蝇的感染。喂食后第3天至第14天间隔24小时。经光学显微镜检查，杜氏疟原虫感染率为47%（n = 258），沙门氏菌感染率为5%（n = 162）。schwetzi。从无鞭毛体到“前环”前鞭毛体的转化发生在两个物种中，在6-18小时后喂养。在杜博斯奇疟原虫中，寄生虫迅速繁殖，并发展成多达10种形式，包括至少3种分裂前鞭毛体形式。后环前鞭毛体，“感染”的形式，出现在6天后喂养，首先在该地区的口膜阀，然后在咽，食钡和长鼻。在喂食后14天的一次尝试中，一只杜氏原蜱传播了L。主要是老鼠咬。相反，寄生虫在S. schwetzi，并没有发展超越“procyclic”promastigotes。寄生虫不能向前迁移，也不能存活超过90小时，表明S。schwetzi不是L的向量。少校从野生捕获的非载体中分离出非感染性早期发育形式的利什曼原虫可能会混淆传统的载体入罪策略。
The extrinsic development of Leishmania major was observed in 2 man-biting sand flies, Phlebotomus duboscqi, a known vector, and Sergentomyia schwetzi, an assumed non-vector. Flies fed on a leishmanial lesion on the nose of a hamster were examined for infection at 0, 6, 12, 18, 24, 36, 48, and 60 hr and at .apprx. 24 hr intervals from day 3 to day 14 post-feeding. Infection rates, determined by light microscopy, were 47% (n = 258) in P. duboscqi and 5% (n = 162) in S. schwetzi. Transformation from amastigotes to "procyclic" promastigotes occurred in both species at 6-18 hr post-feeding. In P. duboscqi, the parasites multiplied rapidly and developed through as many as 10 forms, including at least 3 dividing-promastigote forms. Metacyclic promastigotes, the "infective" form, appeared at 6 days post-feeding, first in the region of the stomodeal valve, then in the pharnyx, cibarium and proboscis. In a single attempt 14 days post-feeding, a P. duboscqi transmitted L. major to a mouse by bite. In contrast, the parasites multiplied slowly in S. schwetzi, and did not develop beyond "procyclic" promastigotes. The parasites did not migrate anteriorly nor survive beyond 90 hr post-feeding, indicating that S. schwetzi is not a vector of L. major. Classical strategies for vector incrimination may be confounded by the isolation of non-infective early developmental forms of Leishmania from wild-caught non-vectors.