In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way
In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way
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DOI：
10.1016/j.carbpol.2017.12.020
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发表时间：
2018-03-01
影响因子：
11.2
通讯作者：
Iacomini, Marcello
中科院分区：
文献类型：
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作者：
Cantu-Jungles, Thaisa Moro;Ruthes, Andrea Caroline;Iacomini, Marcello
膳食纤维的化学和物理结构可能对于理解它们如何调节肠道细菌组成至关重要。我们从 Cookeina spiosa 中纯化了不溶性聚合物，并在体外人类粪便发酵模型中研究了其发酵情况。获得了两种葡聚糖，其特征为(1→3)、(1→6)-连接和(1→3)-连接β-D-葡聚糖。这两种葡聚糖在体外粪便发酵过程中都具有高丁酸和丙酸生成性，气体产生量低，与低聚果糖相比，会导致明显的细菌变化。观察到来自梭状芽孢杆菌 XIVa 簇的拟杆菌和属的特异性增加，例如产丁酸的 Anaerostipes 和 Roseburia。与支链 (1 -> 3),(1 -> 6) 连接 β-D-葡聚糖相比，(1 -> 3)-连接 β-D-葡聚糖呈现出更快的发酵特性。我们的研究结果支持这样的观点，即根据其精细化学结构以及可能的不溶性性质，这些膳食纤维可用于定向促进肠道微生物群向产丁酸梭菌 XIVa 簇细菌生长。
Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition. We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model. Two glucans, characterized as a (1 -> 3),(1 -> 6)-linked and a (1 -> 3)-linked beta-D-glucans were obtained. Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides. Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed. The (1 -> 3)-linked beta-D-glucan presented a faster fermentation profile compared to the branched (1 -> 3),(1 -> 6)-linked beta-D-glucan. Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.