Identification of a novel C-type lectin gene in Japanese flounder, Paralichthys olivaceus.
Identification of a novel C-type lectin gene in Japanese flounder, Paralichthys olivaceus.
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DOI：
10.1016/j.fsi.2007.05.002
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发表时间：
2007-11
影响因子：
4.7
通讯作者：
H. Kondo;Andrew Gung Yeu Tzeh;I. Hirono;T. Aoki
中科院分区：
文献类型：
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作者：
H. Kondo;Andrew Gung Yeu Tzeh;I. Hirono;T. Aoki
在牙鲽肝脏 EST 中鉴定出一种新型牙鲽 C 型凝集素 (JFCTL)。 GenBank 数据库中的 2083 个日本牙鲆肝脏 EST 中，有 48 个与 JFCTL 相同，表明 JFCTL 在肝脏中大量产生。 JFCTL cDNA 包含一个 642bp 的开放阅读框，编码 214 个氨基酸。推导的氨基酸序列中保留了C型凝集素结构域。该蛋白显示出与跨膜型C型凝集素受体的同源性，而不是迄今为止报道的可溶性C型凝集素，但预计它是一种分泌蛋白。 JFCTL 的转录仅限于肝脏。尽管用福尔马林杀死的迟缓爱德华氏菌处理鱼后，mRNA 并未被诱导，但 20°C 下的表达量高于 10°C 下的表达量。
A novel C-type lectin of Japanese flounder (JFCTL) was identified among Japanese flounder liver EST. Of 2083 of Japanese flounder liver ESTs in the GenBank database, 48 were identical to JFCTL, suggesting that JFCTL is abundantly produced in liver. JFCTL cDNA contains an open reading frame of 642bp encoding 214 amino acids. A C-type lectin domain was conserved in the deduced amino acid sequence. The protein showed homology to transmembrane-type C-type lectin receptors rather than the soluble C-type lectin so far reported, but it was predicted to be a secreted protein. Transcription of JFCTL was restricted to the liver. Although the mRNA was not induced after the treatment of fish with formalin-killed Edwardsiella tarda, expression was higher at 20°C than at 10°C.