Accelerated photobleaching of a cyanine dye in the presence of a ternary target DNA, PNA probe, dye catalytic complex: a molecular diagnostic.
Accelerated photobleaching of a cyanine dye in the presence of a ternary target DNA, PNA probe, dye catalytic complex: a molecular diagnostic.
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DOI：
10.1021/ac702519k
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发表时间：
2009-03-15
影响因子：
7.4
通讯作者：
Koshinsky, H.
中科院分区：
文献类型：
--
作者：
Wang, M.;Holmes-Davis, R.;Rafinski, Z.;Jedrzejewska, B.;Choi, K. Y.;Zwick, M.;Bupp, C.;Izmailov, A.;Paczkowski, J.;Warner, B.;Koshinsky, H.
在许多环境中，需要分子测试，但由于复杂性和成本而无法获得，因此我们报告了一种新颖的核酸检测方法。 NA和完整的肽核酸（PNA）探针。 O2，（3）产生的1O2促进Similar cyanine dyes (DiSC3(3), DiSC4(3), DiSC5(3), and DiSCpy(3)) interact with preformed PNA–DNA oligomer duplexes but do not demonstrate an equivalent accelerated photobleaching effect in the presence of PNA and target genomic DNA. The feasibility of developing molecular diagnostic assays based on the accelerated photobleaching (the smartDNA assay) that results from the novel complex正在二线2（3）和PNA – DNA之间形成。
In many settings, molecular testing is needed but unavailable due to complexity and cost. Simple, rapid, and specific DNA detection technologies would provide important alternatives to existing detection methods. Here we report a novel, rapid nucleic acid detection method based on the accelerated photobleaching of the light-sensitive cyanine dye, 3,3′-diethylthiacarbocyanine iodide (DiSC2(3) I−), in the presence of a target genomic DNA and a complementary peptide nucleic acid (PNA) probe. On the basis of the UV–vis, circular dichroism, and fluorescence spectra of DiSC2(3) with PNA–DNA oligomer duplexes and on characterization of a product of photolysis of DiSC2(3) I−, a possible reaction mechanism is proposed. We propose that (1) a novel complex forms between dye, PNA, and DNA, (2) this complex functions as a photosensitizer producing 1O2, and (3) the 1O2 produced promotes photobleaching of dye molecules in the mixture. Similar cyanine dyes (DiSC3(3), DiSC4(3), DiSC5(3), and DiSCpy(3)) interact with preformed PNA–DNA oligomer duplexes but do not demonstrate an equivalent accelerated photobleaching effect in the presence of PNA and target genomic DNA. The feasibility of developing molecular diagnostic assays based on the accelerated photobleaching (the smartDNA assay) that results from the novel complex formed between DiSC2(3) and PNA–DNA is under way.
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影响因子：
2.9
作者：
Hannah, KC;Gil, RR;Armitage, BA
通讯作者：
Armitage, BA
影响因子：
2.9
作者：
Mollegaard, NE;Bailly, C;Nielsen, PE
通讯作者：
Nielsen, PE
影响因子：
3.4
作者：
Biver, T;De Biasi, A;Yarmoluk, S
通讯作者：
Yarmoluk, S
DOI：
10.1016/1011-1344(88)85004-8
发表时间：
1988-05-01
影响因子：
5.4
作者：
HOEBEKE, M;SERET, A;VANDEVORST, A
通讯作者：
VANDEVORST, A
DOI：
10.1073/pnas.96.21.11804
发表时间：
1999-10-12
影响因子：
11.1
作者：
Lohse, J;Dahl, O;Nielsen, PE
通讯作者：
Nielsen, PE