Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.).
Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.).
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DOI：
10.1270/jsbbr.6.143
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Izawa, T.
中科院分区：
文献类型：
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作者：
Sugiura, N.;Tsuji, T.;Izawa, T.
利用分子标记辅助选择（MAS）技术，以水稻细胞质雄性不育系为材料，通过轮回回交选育，获得了分别含有抗条纹叶枯病基因Stvb1和抗穗颈瘟基因Pb1的越光近等基因系。这两个抗病基因连锁在供体St.No.1的第11染色体上，距离为5.2 cM。越光的烹饪品质享有盛誉，但缺乏这两种抗性基因。利用一个与Stvbi连锁在0.0cM以内的分子标记ST 10和4个与Pb 1紧密连锁的分子标记进行了穗瘟抗性的轮回回交，获得了较高的准确率。特别地，通过使用区分纯合子的Pb1的CAPS标记B4获得稻瘟病抗性的早期固定。作者选育出了一个既抗条纹叶枯病又抗穗瘟的越光近等基因系“越光爱知SBL”，该系表现出与越光相似的一系列性状。MAS的效率表现在育种所需的成本和时间方面。
Molecular marker-assisted selection (MAS) was conducted for recurrent backcross breeding with a cytoplasmic male sterility line to develop Koshihikari near-isogenic lines harboring the resistance genes, Stvbi and Pb1, to rice stripe virus and panicle blast respectively. The two resistance genes were linked at a distance of 5.2 cM on chromosome 11 in a donor line St.No.1. Koshihikari enjoys a high reputation for its cooking quality, but lacks the two resistance genes. The recurrent backcross was conducted with a high level of accuracy by using a molecular marker ST10 linked to Stvbi within 0.0 cM and four molecular markers closely linked to Pb1 for the resistance to panicle blast, which can not be easily tested under field conditions. In particular, early fixation of the blast resistance was obtained by using a CAPS marker B4 for Pb1 which differentiated homozygotes. The authors were able to breed a Koshihikari near-isogenic line, "Koshihikari Aichi SBL" that was resistant to both rice stripe virus and panicle blast and displayed a set of characters similar to them of Koshihikari. Efficiency of MAS was demonstrated in terms of cost and time required for the breeding.