Muscle precursor cells injected into irradiated mdx mouse muscle persist after serial injury
Muscle precursor cells injected into irradiated mdx mouse muscle persist after serial injury
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DOI：
10.1002/(sici)1097-4598(199902)22:2
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发表时间：
1999-02-01
期刊：
影响因子：
3.4
通讯作者：
Morgan, JE
中科院分区：
文献类型：
--
作者：
Gross, JG;Morgan, JE
将H-2K(b)-tsA58肌肉前体细胞（mpc）植入辐照的mdx nu/nu小鼠肌肉中，形成供体来源的肌肉。肌毒素诺特辛（notexin）可以破坏成熟的肌纤维，但保留肌肉前体细胞和其他组织。在注射mpc的肌肉中注射了一系列的肌毒素，在每次注射之间留出了再生的时间。在四次诺特辛治疗后，供体来源的新肌纤维形成，这提供了证据，表明一些植入的mpc重新进入未分化的、静止的、干细胞样的状态，并且在肌肉进一步损伤后能够形成肌肉。一个类似的模型可以用来测定人类mpc制剂在植入患者之前是否含有持久的前体细胞。在对照mdx肌肉中，经过辐照，注射组织培养基，并注射三次诺特辛，也发生了再生，表明存在抗辐射mpc，可能是在处理过的肌肉中。(C) 1999 John Wiley & Sons, Inc
Muscle of donor origin was formed after implantation of H-2K(b)-tsA58 muscle precursor cells (mpc) into irradiated mdx nu/nu mouse muscles. A series of injections of the myotoxin, notexin, which destroys mature muscle fibers but spares muscle precursor cells and other tissues, was made into the mpc-injected muscles, leaving time for regeneration to occur between each injection. New muscle fibers of donor origin were formed after up to four notexin treatments, providing evidence that some of the implanted mpc reentered an undifferentiated, quiescent, stem cell-like state and were capable of myogenesis after further injuries to the muscle. A similar model could be used to assay whether preparations of human mpc contain long-lasting precursor cells, prior to their implantation into patients. In control mdx muscles, which had been irradiated, injected with tissue culture medium, and given three notexin injections, regeneration also occurred, indicating that radiation-resistant mpc were present, presumably within the treated muscle. (C) 1999 John Wiley & Sons, Inc.