Host-induced epidemic spread of the cholera bacterium
Host-induced epidemic spread of the cholera bacterium
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DOI：
10.1038/nature00778
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发表时间：
2002-06-06
期刊：
影响因子：
64.8
通讯作者：
Camilli, A
中科院分区：
文献类型：
--
作者：
Merrell, DS;Butler, SM;Camilli, A
在流行病传播过程中，增强病原体传播的因素尚未明确。霍乱的水传播在自然界中迅速发生，而在没有胃酸缓冲的情况下，用体外培养的细菌感染人类志愿者是困难的（1）。然而，目前还不清楚胃酸是否是导致流行病传播的主要因素（2）。在这里，我们报告说，表征霍乱弧菌从人类粪便支持一个模型，即人类殖民创造一个高感染性的细菌状态，传播后保持，可能有助于霍乱的流行传播。粪便样本中霍乱弧菌的转录谱显示了一种独特的生理和行为状态，其特征在于营养获取和运动所需基因的高表达水平，以及细菌趋化性所需基因的低表达水平。
The factors that enhance the transmission of pathogens during epidemic spread are ill defined. Water-borne spread of the diarrhoeal disease cholera occurs rapidly in nature, whereas infection of human volunteers with bacteria grown in vitro is difficult in the absence of stomach acid buffering(1). It is unclear, however, whether stomach acidity is a principal factor contributing to epidemic spread(2). Here we report that characterization of Vibrio cholerae from human stools supports a model whereby human colonization creates a hyperinfectious bacterial state that is maintained after dissemination and that may contribute to epidemic spread of cholera. Transcriptional profiling of V. cholerae from stool samples revealed a unique physiological and behavioural state characterized by high expression levels of genes required for nutrient acquisition and motility, and low expression levels of genes required for bacterial chemotaxis.