Granulomatous peritonitis and pleuritis in interferon-γ gene knockout mice naturally infected with mouse hepatitis virus
Granulomatous peritonitis and pleuritis in interferon-γ gene knockout mice naturally infected with mouse hepatitis virus
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DOI：
10.1111/j.1751-0813.1999.tb13199.x
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发表时间：
1999-09-01
影响因子：
1.1
通讯作者：
Barthold, SW
中科院分区：
文献类型：
--
作者：
France, MP;Smith, AL;Barthold, SW
目的通过尸检、组织病理学、血清学和免疫组化等方法，对一起干扰素γ（IFN-γ）基因突变引起的实验鼠疾病暴发进行研究。尸检显示肉芽肿性腹膜炎和胸膜炎伴广泛粘连，但实质病变极轻微。血清标本中有高浓度的抗小鼠肝炎病毒抗体，免疫组化检查显示肉芽肿巨噬细胞中存在小鼠肝炎病毒抗原。其他感染因子和细菌培养物的血清学检测均为阴性，同一设施中饲养的野生型小鼠保持健康。尽管该疾病与小鼠肝炎病毒感染之间存在关联，但小鼠肝炎病毒所起的确切作用尚未确定。虽然这种疾病是表面上类似于猫传染性腹膜炎（另一种冠状病毒引起的浆膜炎），差异存在于这两个conditions.Conclusion的组织病理学结果，这种不寻常的疾病过程说明了如何新的诊断挑战，可以出现在新的小鼠基因型通过分子遗传学。此外，该疾病与小鼠肝炎病毒之间的关联说明了在无特定病原体的条件下维持实验室动物的重要性。
Objective To investigate a disease outbreak in a colony of laboratory mice with targeted disruption of the gene for interferon-gammaFormat A case report based on necropsy, histopathology, serology and immunohistochemistry.Results Affected mice exhibited depression and variable ascites. Necropsy revealed a granulomatous peritonitis and pleuritis with extensive adhesions although parenchymal lesions were minimal. Serum samples had high concentrations of antibody to mouse hepatitis virus and immunohistochemical examination revealed the presence of mouse hepatitis virus antigen in granuloma macrophages. Sere-logical testing for other infectious agents and bacterial culture were negative and wild type mice kept in the same facility remained healthy. Despite the association between the disease and mouse hepatitis virus infection, the precise role played by mouse hepatitis virus was not determined. While the disease is superficially similar to feline infectious peritonitis (another coronavirus-induced serositis), differences exist between the histopathological findings in these two conditions.Conclusion This unusual disease process illustrates how new diagnostic challenges can arise in novel mouse genotypes created through molecular genetics. Furthermore, the association between the disease and mouse hepatitis virus illustrates the importance of maintaining laboratory animals under specific-pathogen free conditions.