Biocontrol Potential of a Lytic Bacteriophage PE204 against Bacterial Wilt of Tomato
Biocontrol Potential of a Lytic Bacteriophage PE204 against Bacterial Wilt of Tomato
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DOI：
10.4014/jmb.1208.08072
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Lee, Seon-Woo
中科院分区：
文献类型：
--
作者：
Bae, Ju Young;Wu, Jing;Lee, Seon-Woo
青枯病是由青枯菌（Ralstonia solanacearum）引起的一种重要的经济作物病害。由于目前还没有有效的防治青枯病的策略，因此可以采用噬菌体疗法，即使用强毒噬菌体。以噬菌体PE 204为模式裂解性噬菌体，研究其对番茄青枯病的生防潜力。噬菌体PE 204具有与短尾病毒科成员相似的短尾二十面体结构和双链DNA基因组。PE 204在宽的温度和pH范围内稳定，并且在表面活性剂Silwet L-77存在下也稳定。采用人工土壤微宇宙（ASM）研究噬菌体在土壤中的稳定性，在受控系统下研究噬菌体的活力。尽管噬菌体在ASM中在高温下显示出较低的稳定性，但宿主细菌的存在有助于维持稳定的噬菌体群体。用10（8）PFU/ml的噬菌体PE 204与R.番茄根际青枯菌完全抑制青枯病的发生，向噬菌体悬浮液中添加0.1%的Silwet L-77不会损害PE 204的防病活性。在番茄根际施用噬菌体PE 204后，在番茄根际施用噬菌体PE 204后，对番茄根际细菌的生防活性明显高于施用前。还研究了青枯菌接种。然而，用噬菌体预处理对青枯病的防治无效，后处理的PE 204延迟青枯病的发展。我们的研究结果表明，在Silwet L-77等表面活性剂的作用下，将溶菌噬菌体适当施用到植物根系中可以用于防治作物青枯病。
Bacterial wilt caused by Ralstonia solanacearum is a devastating disease of many economically important crops. Since there is no promising control strategy for bacterial wilt, phage therapy could be adopted using virulent phages. We used phage PE204 as a model lytic bacteriophage to investigate its biocontrol potential for bacterial wilt on tomato plants. The phage PE204 has a short-tailed icosahedral structure and double-stranded DNA genome similar to that of the members of Podoviridae. PE204 is stable under a wide range of temperature and pH, and is also stable in the presence of the surfactant Silwet L-77. An artificial soil microcosm (ASM) to study phage stability in soil was adopted to investigate phage viability under a controlled system. Whereas phage showed less stability under elevated temperature in the ASM, the presence of host bacteria helped to maintain a stable phage population. Simultaneous treatment of phage PE204 at 10(8) PFU/ml with R. solanacearum on tomato rhizosphere completely inhibited bacterial wilt occurrence, and amendment of Silwet L-77 at 0.1% to the phage suspension did not impair the disease control activity of PE204. The biocontrol activities of phage PE204 application onto tomato rhizosphere before or after R. solanacearum inoculation were also investigated. Whereas pretreatment with the phage was not effective in the control of bacterial wilt, post-treatment of PE204 delayed bacterial wilt development. Our results suggested that appropriate application of lytic phages to the plant root system with a surfactant such as Silwet L-77 could be used to control the bacterial wilt of crops.