POLYKARYOCYTE FORMATION INDUCED BY VSV IN MOUSE-L CELLS
POLYKARYOCYTE FORMATION INDUCED BY VSV IN MOUSE-L CELLS
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DOI：
10.1099/0022-1317-32-1-85
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发表时间：
1976-01-01
影响因子：
3.8
通讯作者：
NAGATA, I
中科院分区：
文献类型：
--
作者：
NISHIYAMA, Y;ITO, Y;NAGATA, I
用VSV [水泡性口炎病毒]感染小鼠[成纤维细胞] L细胞导致多核细胞的形成。4-12 h p.i. [感染后]。当在感染过程中加入[兔]抗VSV免疫血清时，细胞融合的进展很快被抑制。放线菌酮在注射后1 h内加入可完全抑制细胞融合;当在3小时和3小时后加入药物时，细胞融合完全没有受到抑制。早期的多核细胞形成，融合，从没有，只观察到在非常高的多重性感染的细胞在低水平。VSV诱导的细胞融合在几种细胞类型中的发展是不同的，尽管所有这些细胞都产生了相当高的病毒产量：L和C-243-3小鼠细胞系显示出高水平的多核细胞（80-100%），BHK [幼仓鼠肾]和RK-13 [兔肾-13]细胞的反应水平较低; PS [猪肾]和Vero [绿色猴肾]细胞对VSV感染没有表现出细胞融合。在PS细胞中，当VSV感染的L细胞共培养时发生细胞融合。并对VSV诱导细胞融合的机制进行了讨论。
Infection of mouse [fibroblast] L cells with VSV [vesicular stomatitis virus] leads to the formation of polykaryocytes .apprx. 4-12 h p.i. [post-infection]. When [rabbit] anti-VSV immune serum was added during the course of infection, progression of cell fusion was soon suppressed. Cycloheximide completely suppressed the cell fusion when the drug was added within 1 h p.i.; cell fusion was not suppressed at all when the drug was added at and after 3 h. Early polykaryocyte formation, fusion from without, was observed only at a low level in cells infected at very high multiplicities. The development of cell fusion induced by VSV was different in several cell types, although all these cells produced a rather high yield of virus: L and C-243-3 mouse cell lines showed a high level of polykaryocytosis (80-100%); BHK [baby hamster kidney] and RK-13 [rabbit kidney-13] cells responded at low level; and PS [porcine kidney] and Vero [green monkey kidney] cells showed no cell fusion in response to VSV infection. In PS cells, cell fusion occurred when VSV-infected L cells were co-cultivated. The mechanism of cell fusion induced by VSV was discussed.