Microfluidic Characterization and Continuous Separation of Cells and Particles Using Conducting Poly(dimethyl siloxane) Electrode Induced Alternating Current-Dielectrophoresis
Microfluidic Characterization and Continuous Separation of Cells and Particles Using Conducting Poly(dimethyl siloxane) Electrode Induced Alternating Current-Dielectrophoresis
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DOI：
10.1021/ac202137y
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发表时间：
2011-12-15
影响因子：
7.4
通讯作者：
Stocker, Roman
中科院分区：
文献类型：
--
作者：
Lewpiriyawong, Nuttawut;Kandaswamy, Kumaravel;Stocker, Roman
本文提出了一种聚二甲基硅氧烷（PDMS）聚合物微流控装置，使用交流（ac）介电电泳（DEP）分离活细胞从类似大小的干扰颗粒的极化率在连续流动和表征DEP行为的细胞在停滞流。ac-DEP力由在装置主通道的侧壁上制造的三维（3D）导电PDMS复合电极产生。这种3D PDMS复合电极是通过将微米级银（Ag）填料分散到PDMS凝胶中制成的。侧壁AgPDMS电极可以产生在整个沟道高度上均匀分布并且沿着沟道横向变化的3D电场，从而在整个沟道上产生更强的横向DEP效应。这不仅允许容易地观察细胞/颗粒的横向运动，而且允许使用横向DEP力来操纵细胞/颗粒。前一个特征用于表征酿酒酵母（酵母）和大肠杆菌（细菌）的频率依赖性DEP行为。后者用于从相似大小的乳胶颗粒中连续分离活酵母和细菌细胞，以及从死酵母细胞中连续分离活酵母细胞。在所有情况下都实现了97%的分离效率。这些功能的演示显示了微流控器件的应用前景。
This paper presents a poly(dimethyl siloxane) (PDMS) polymer microfluidic device using alternating current (ac) dielectrophoresis (DEP) for separating live cells from interfering particles of similar sizes by their polarizabilities under continuous flow and for characterizing DEP behaviors of cells in stagnant flow. The ac-DEP force is generated by three-dimensional (3D) conducting PDMS composite electrodes fabricated on a sidewall of the device main channel. Such 3D PDMS composite electrodes are made by dispersing microsized silver (Ag) fillers into PDMS gel. The sidewall AgPDMS electrodes can generate a 3D electric field that uniformly distributes throughout the channel height and varies along the channel lateral direction, thereby producing stronger lateral DEP effects over the entire channel. This allows not only easy observation of cell/particle lateral motion but also using the lateral DEP force for manipulation of cells/particles. The former feature is used to characterize the frequency-dependent DEP behaviors of Saccharomyces cerevisiae (yeast) and Escherichia coli (bacteria). The latter is utilized for continuous separation of live yeast and bacterial cells from similar-size latex particles as well as live yeast cells from dead yeast cells. The separation efficiency of 97% is achieved in all cases. The demonstration of these functions shows promising applications of the microfluidic device.