RESISTANCE TO AMERICAN FOULBROOD IN HONEY BEES .1. DIFFERENTIAL SURVIVAL OF LARVAE OF DIFFERENT GENETIC LINES
RESISTANCE TO AMERICAN FOULBROOD IN HONEY BEES .1. DIFFERENTIAL SURVIVAL OF LARVAE OF DIFFERENT GENETIC LINES
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DOI：
10.1093/jee/49.4.470
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发表时间：
1956-01-01
影响因子：
2.2
通讯作者：
THOMPSON, VC
中科院分区：
文献类型：
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作者：
ROTHENBUHLER, WC;THOMPSON, VC
对意大利蜜蜂（Apis mellifera L.）在通过特制的微量注射器施用在0.25立方毫米水中的50，000个芽孢杆菌幼虫白色孢子接种幼虫食物后，显示出三种不同的存活百分比。为了从不同的蜂王获得相似年龄的幼虫，将它们关在类似的梳子上，持续已知的小时数，之后将所有含有卵的测试梳子放入商业杂交蜂的单个保育群中。在幼虫生命的第1天进行接种。对照幼虫，给予不含孢子的水，在每个线中显示超过95%的存活率。幼虫孢子在感病品系中的存活率为25%，在抗虫品系中的存活率为47%，在一般抗性品系中的存活率为67%。存活率的这些差异非常显著，并且它们被解释为是由于3个品系的幼虫中对美洲腐殖幼虫的不同水平的先天抗性。
Larvae of three genetically distinct lines of honey bees (Apis mellifera L.) showed three different percentages of survival following inoculations of larval food with 50,000 spores of Bacillus larvae White in 0.25 cubic millimeter of water administered by a specially made micro-syringe. In order to obtain larvae of similar age from the various queens, they were caged on similar combs for a known number of hours, after which all test combs containing eggs were put into a single nurse colony of commercial hybrid bees. Inoculations were made during the 1st day of larval life. Check larvae, given water containing no spores, showed survival in excess of 95% in each line. Larvae given spores survived to the extent of 25% in a line selected and bred for susceptibility, 47% in a line selected for larval resistance for 1 generation by a special technique, and 67% in a line selected and bred for general resistance. These differences in survival are very highly significant and they are interpreted to be due to different levels of innate resistance to American foulbrood in the larvae of the 3 lines.