GARP is highly conserved among Trypanosoma congolense Savannah, Forest and Kilifi subgroups
GARP is highly conserved among Trypanosoma congolense Savannah, Forest and Kilifi subgroups
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DOI：
10.1016/s0166-6851(99)00217-0
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发表时间：
2000-03-05
影响因子：
1.5
通讯作者：
Gibson, W
中科院分区：
文献类型：
--
作者：
Asbeck, K;Ruepp, S;Gibson, W
在布氏锥虫中，原环素是昆虫期的主要表面糖蛋白。在昆虫形式的T中已经鉴定出一种前环素形式的功能类似物。（Nannomonas）congolense;这与原环素几乎没有序列同源性，并被命名为富含谷氨酸/丙氨酸的蛋白（GARP）。以前的研究结果表明，GARP在刚果锥虫中是保守的，而在南单胞菌亚属的另外两个成员：刚果锥虫和刚果锥虫中不保守。simiaeandT.戈弗雷。以基因组DNA为模板进行聚合酶链反应，以确定T的萨凡纳、Forest和Kilifi亚群中GARP序列的保守程度。刚果语。GARP基因在刚果锥虫中高度保守，但在整个南单胞菌亚属中并不保守。这表明，该亚属在昆虫媒介中的特征性中肠-喙发育周期不依赖于GARP。
InTrypanosoma(Trypanozoon)brucei, procyclins are the major surface glycoproteins in the insect stage. A functional analogue of one form of procyclin has been identified in insect forms ofT.(Nannomonas)congolense; this shares virtually no sequence homology with procyclin and has been named glutamic acid/alanine-rich protein (GARP). Previous results have indicated thatGARPis conserved inT. congolense, but not in the 2 other members of the subgenusNannomonas:T. simiaeandT. godfreyi. Genomic DNA was used as a template in the polymerase chain reaction to ascertain the extent ofGARPsequence conservation in the Savannah, Forest and Kilifi subgroups ofT. congolense. It was concluded thatGARPis well-conserved inT. congolense, but not in the whole of the subgenusNannomonas. This suggests that the characteristic midgut-proboscis development cycle of this subgenus in the insect vector is not dependent on GARP.