Isolation, characterization, and chromosomal mapping of the human Nkx6.1 gene (NKX6A), a new pancreatic islet homeobox gene
Isolation, characterization, and chromosomal mapping of the human Nkx6.1 gene (NKX6A), a new pancreatic islet homeobox gene
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DOI：
10.1006/geno.1996.4568
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发表时间：
1997-03-01
期刊：
影响因子：
4.4
通讯作者：
Permutt, MA
中科院分区：
文献类型：
--
作者：
Inoue, H;Rudnick, A;Permutt, MA
Nkx6.1（基因符号MKX 6A）是最近在啮齿动物胰岛β细胞系中鉴定出的NK同源异型盒基因家族的新成员。表达模式表明，该基因产物可能是重要的控制胰岛发育和/或调节胰岛素生物合成。我们现在报告克隆的人NKX 6A，其基因组结构的表征，其染色体定位。预测的人NKX 6A蛋白含有367个氨基酸，与仓鼠蛋白的同源性为97%。高度保守的NK十肽和同源结构域区域在人和仓鼠之间是相同的，表明这些结构域的功能重要性。编码区全长约4.8kb，由3个外显子组成。将该基因定位于4个CEPH“B”型酵母人工染色体克隆（914 B4、951 G9、981 D 6和847 B3），并在第4染色体上发现了一个邻近的多态性标记（D4 S1538
Nkx6.1 (gene symbol MKX6A), a new member of the NK homeobox gene family, was recently identified in rodent pancreatic islet beta-cell lines. The pattern of expression suggested that this gene product might be important for control of islet development and/or regulation of insulin biosynthesis. We now report cloning of human NKX6A, characterization of its genomic structure, and its chromosomal localization. The predicted protein of human NKX6A contained 367 amino acids and had 97% identity to the hamster protein. The highly conserved NK decapeptide and homeodomain regions were identical between human and hamster, suggesting functional importance of these domains. The coding region spanned approximately 4.8 kb and was composed of three exons. The gene was localized to four CEPH ''B'' yeast artificial chromosome clones (914B4, 951G9, 981D6, and 847B3), and a nearby polymorphic marker (D4S1538) on chromosome 4 was identified